ArticleMicrobial biotechnology2026
Identification of the Integration/Excision Module and Regulatory Elements Involved in the Mobility of IME8, an Integrative and Mobilizable Element From Mosquitocidal Lysinibacillus sphaericus.
Article in Microbial biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Lysinibacillus sphaericus, a bacterium successfully used in the control of mosquitoes, bears its insecticidal traits in GI8, a recently identified mosquitocidal genomic island. GI8 is renamed IME8 in the present work, as it displays a typical genetic organization of an Integrative and Mobilizable Element (IME) and its circularized form is not self-conjugative but mobilizable by the pBsph-like plasmid p1593. The IME8 integration module (int-operon) encodes two integrase-like proteins (Int1 and Int2) belonging to the family of tyrosine recombinases, and a hypothetical protein (Hp3). All three ORFs are necessary and function as an essential excision unit of IME8. The chimeric construct "attL-int1-int2-hp3-kan-attR" (hereafter named mini-IME8 cassette) displays integrating property. The integration is specific to an acnL-yolD(attB)-uvrX operon target region, which is not only distributed in various L. sphaericus isolates but is also present among other Lysinibacillus species. The regulation module, reg-operon, encodes an HTH-domain-carrying protein (Reg16) and a putative lytic polysaccharide monooxygenase (LPMO17). Knockout of the reg-operon remarkably increases IME8 excision and transcription levels of int1/int2/hp3 compared to the wild-type situation. However, expression of reg16 or the complete reg-operon both increase the int-operon promoter (P
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.