ArticleApplied microbiology and biotechnology2026
Impact of direct cell-cell contact in a three-species wine yeast consortium.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wine fermentations provide a model ecosystem for studying microbial interactions between yeast species. This includes antagonistic, neutral and synergistic interactions, in which nutrient competition, metabolic exchange, and killer toxin production were identified as factors impacting ecosystem functioning. Several studies have shown that physical contact between cells of different species also affects ecosystem functioning; however, the mechanisms by which cell-cell contact elicits such responses remains poorly understood. The molecular impact of physical contact was evaluated using membrane bioreactors to physically separate species while allowing metabolic exchange. However, bioreactor designs have been limited in such studies to evaluating two-species binary interactions. Therefore, we evaluated the impact of direct cell-cell contact on the physiology and gene expression of Saccharomyces cerevisiae within a yeast consortium including Lachancea thermotolerans and Torulaspora delbrueckii. A membrane bioreactor allowed continuous media exchange between separate fermentation vessels. Yeast growth, extracellular metabolites, and S. cerevisiae transcriptional profiles were monitored at two timepoints. Direct cell contact favored S. cerevisiae growth and resulted in significantly different amino acid profiles, while indirect (metabolic) contact favored T. delbrueckii growth at the expense of L. theromotolerans. In addition, transcriptional analysis revealed that treatment-specific gene regulation differed from responses previously reported for two-species systems of these yeasts, e.g., strong induction of HPF1, while similarities included upregulation of high-affinity hexose transporters (e.g., HXT6 and 7) and downregulation of sporulation genes. These results indicate that cell-cell contact impacts ecosystem dynamics and nutrient uptake and promotes distinct gene expression responses in S. cerevisiae within a multispecies wine consortium. KEY POINTS: • Multispecies consortium moves beyond binary yeast interaction studies. • S. cerevisiae growth and amino acid profiles differ in direct and indirect contact. • Direct contact elicits unique S. cerevisiae gene expression, e.g., HPF1 upregulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.