ArticleBMC microbiology2026
Influence of biological pretreatment of coconut husk lignocellulosic biomass for biogas production on archaeal community and volatile fatty acids.
Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Lignocellulosic agricultural residues such as coconut husk are abundant but recalcitrant substrates that limit efficient biogas production during anaerobic digestion. We evaluated the effect of biological pretreatment of coconut husk on archaea, volatile fatty acid (VFA) profiles, and biogas production during anaerobic digestion. Coconut husk was pretreated for 50 days using a consortium of cellulolytic bacteria isolated from poultry feces and subsequently digested alongside untreated husk (control) in batch reactors. Cellulose-degrading bacteria were presumptively characterized based on morphological, cultural, and biochemical characteristics and included Bacillus megaterium, Bacillus licheniformis, Lactobacillus plantarum, Bacillus cereus, and Bacillus subtilis based on morphological, cultural, and biochemical characteristics. Ten archaeal genera were detected across treatments, including Methanobrevibacter, Methanomassiliicoccus, Methanobacterium, Methanoculleus, Methanosarcina, Methanomicrobium, Desulfurococcus, Thermosphaera, Methanogenium, and Methanoregula. The control digestate exhibited higher archaeal diversity with a low methane production. The pretreated reactor (Ch + Cdb) produced a cumulative biogas yield of 88,355 mL gVS⁻
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