ArticleNature methods2026
A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.
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Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9citing papers in PubMed
–field-weighted citation impact
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Who cites it
9 citing papers in PubMed.
- OpticalFlow3D - a tool for measuring amorphous motion in three-dimensional fluorescence microscopy images.Journal of cell science · 2026Article
- Closing the Loop: High-Precision 3D Photofabrication in Living Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A custom two-in-one HIST and line-scanning confocal excitation module.bioRxiv : the preprint server for biology · 2026Article
- Lipid-driven membrane remodeling engages a lysosome-dependent adaptive repair program during retinal aging.Molecular neurodegeneration · 2026Article
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- Simulation of transcription factor clustering in nuclei from molecular kinetics.bioRxiv : the preprint server for biology · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- Age-Driven Lipid Remodeling Activates Lysosome-Mediated Plasma Membrane Repair.Research square · 2026Article
- Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning.Science (New York, N.Y.) · 2025Article
Corrections and comments
- Update of
Authors and funding
49 authors.
Tian-Ming Fu *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0001-6265-0859
Gaoxiang Liu *Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Daniel E Milkie *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0002-3917-6965
Xiongtao Ruan *Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0001-5322-3249
Frederik GörlitzDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-8415-1820
Yu ShiLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID http://orcid.org/0000-0002-8827-7500
Valentina FerroDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-9891-6468
Nikita S DivekarDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0009-0006-9384-8407
Wei WangDepartment of Chemistry, University of Illinois Chicago, Chicago, IL, USA.
Harrison M YorkMonash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0002-4534-8188
Velat KilicJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Matthew MuellerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0003-0774-1031
Yajie LiangDepartment of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Timothy A DaugirdDepartment of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.ORCID http://orcid.org/0000-0002-6313-0246
Maria Jose Gacha-GarayDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.
Kathryn A LarkinDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.
Rebecca C AdikesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.ORCID http://orcid.org/0000-0002-7526-8701
Nathanael HarrisonDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Cyna ShirazinejadDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Samara WilliamsDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Jamison L NourseDepartment of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, USA.
Shu-Hsien SheuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0003-0758-4654
Liang GaoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Tongchao LiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Chandrani MondalDepartment of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Kemal AchourDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Wilmene HerculeDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Daniel R StableyNeuroimaging Laboratory, Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Kevin EmmerichWilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Peng DongJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0003-4946-1409
David G DrubinDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Zhe J LiuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.ORCID http://orcid.org/0000-0002-3592-3150
Jeff S MummWilmer Eye Institute and the Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-2575-287X
Minoru KoyamaDepartment of Cell and Systems Biology, University of Toronto, Scarborough, Ontario, Canada.ORCID http://orcid.org/0000-0002-9774-9223
Alison N KillileaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-8308-8196
Jose Javier Bravo-CorderoDepartment of Medicine, Division of Hematology and Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0001-9481-1231
C Dirk KeeneUniversity of Washington BioRepository and Integrated Neuropathology (BRaIN) laboratory, Harborview Medical Center, Seattle, WA, USA.ORCID http://orcid.org/0000-0002-5291-1469
Liqun LuoDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-5467-9264
Tomas KirchhausenProgram in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.ORCID http://orcid.org/0000-0003-0559-893X
Medha M PathakDepartment of Physiology and Biophysics, Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0002-6518-3085
Senthil ArumugamMonash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton/Melbourne, Victoria, Australia.ORCID http://orcid.org/0000-0001-6733-4679
James K NuñezDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-7396-6119
Ruixuan GaoDepartment of Chemistry, University of Illinois Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0001-7137-2512
David Q MatusDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.
Benjamin L MartinDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, USA.
Ian A SwinburneDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Eric BetzigJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. betzige@janelia.hhmi.org.ORCID http://orcid.org/0000-0002-4192-1274
Wesley R LegantLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. legantw@email.unc.edu.ORCID http://orcid.org/0000-0003-1827-5371
Srigokul UpadhyayulaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. sup@berkeley.edu.ORCID http://orcid.org/0000-0001-6727-5883
Funding
Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI JEFFREY MUMM · 1985 to 2026
$22.1MNikon confocal system for improved spatio-dynamic analysis of endocytic trafficking eventsR35GM118149 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI DAVID G DRUBIN · 2016 to 2026
$12.0MTRAINING PROGRAM IN CANCER THERAPYT32CA078207 · NCI · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI James J Manfredi · 1999 to 2026
$11.6MVISUALIZATION OF SUBCELLULAR DYNAMICS IN MULTICELLULAR ORGANISMSR35GM130386 · NIGMS · BOSTON CHILDREN'S HOSPITAL · PI TOMAS KIRCHHAUSEN · 2019 to 2026
$4.0MBuilding the brain: How mechanical forces shape human neural developmentDP2AT010376 · NCCIH · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2020
$2.9MPiezo1 in neural stem cell mechano-regulationR01NS109810 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PATHAK, MEDHA M · 2018 to 2022
$2.2MNovel models of Alzheimer's Disease and Related Disorders enabling systematic investigation of the therapeutic potential of transplanted hiPSC-derived cells and endogenous adult neural stem cellsR01OD020376 · OD · JOHNS HOPKINS UNIVERSITY · PI MUMM, JEFFREY · 2016 to 2020
$2.1MElucidating the mechanisms underlying cell cycle regulation of invasive behaviorR01GM121597 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI MATUS, DAVID · 2017 to 2021
$2.1MDefining the role of type III collagen and the collagen-binding receptor DDR1 in metastatic dormancyR01CA244780 · NCI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BRAVO-CORDERO, JOSE JAVIER · 2020 to 2024
$1.9MMolecular and Cell Biology of Neuromesodermal ProgenitorsR35GM150290 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI Benjamin L Martin · 2023 to 2026
$1.7MInnate immune system regulation of retinal regenerationR01EY033009 · NEI · JOHNS HOPKINS UNIVERSITY · PI MUMM, JEFFREY · 2022 to 2024
$1.3MCytoskeletal and Cell Cycle Regulation of Cell Migration During DevelopmentF32GM133131 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI ADIKES, REBECCA C · 2019 to 2020
$126kDOE | LDRD | Lawrence Berkeley National Laboratory (Berkeley Lab) LDRD 7647437 and 7721359NCI NIH HHS R01 CA244780NCI NIH HHS T32 CA078207NEI NIH HHS F31 EY032790NEI NIH HHS P30 EY001765NEI NIH HHS R01 EY033009NIGMS NIH HHS R35 GM130386NIH HHS R01 OD020376U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1DP2GM136653U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1R01DC021710U.S. Department of Health & Human Services | National Institutes of Health (NIH) DP2AT010376U.S. Department of Health & Human Services | National Institutes of Health (NIH) F32GM133131U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01CA244780U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01‑DC005982U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01GM121597U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01NS109810U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01OD020376U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35GM118149U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35GM150290U.S. Department of Health & Human Services | National Institutes of Health (NIH) T32EY7143‑22U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) P30‑CA196521U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) GM130386
Abstract
Understanding biological systems requires observing features and processes across vast spatial and temporal scales, spanning nanometers to centimeters and milliseconds to days, often using multiple imaging modalities within complex native microenvironments. Yet, achieving this comprehensive view is challenging because microscopes optimized for specific tasks typically lack versatility due to inherent optical and sample handling tradeoffs, and frequently suffer performance degradation from sample-induced optical aberrations in multicellular contexts. Here, we present Multimodal Optical Scope with Adaptive Imaging Correction (MOSAIC), a reconfigurable microscope that integrates multiple advanced imaging techniques including light-sheet, label-free, super-resolution and multiphoton, all equipped with adaptive optics. MOSAIC enables noninvasive imaging of subcellular dynamics in both cultured cells and live multicellular organisms, nanoscale mapping of molecular architectures across millimeter-scale expanded tissues and structural/functional neural imaging within live mice. MOSAIC facilitates correlative studies across biological scales within the same specimen, providing an integrated platform for broad biological investigation.
Indexed as
MicroscopyMultimodal ImagingAnimalsHumansMiceMicroscopy, Fluorescence, Multiphoton
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