ArticleHistochemistry and cell biology2026
A rapid and robust translational model for testicular aging research.
Article in Histochemistry and cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- June in focus in HCB: advanced methodologies.Histochemistry and cell biology · 2026Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
In humans, testicular peritubular cells (TPCs) form a small compartment surrounding the seminiferous tubules and, as shown previously, undergo age-related changes. How they may contribute to the age-associated decline of testicular functions is not well known. Likewise, the mechanisms of testicular aging in humans are not well examined. This is in part due to the lack of appropriate cellular models. We aimed to establish an aging model of TPCs, which is based on immortalized nonhuman primate (NHP) peritubular cells (iMKTPCs) from Callithrix jacchus. As shown previously by a comprehensive proteomic approach, they strongly resemble human TPCs but lack the human cell-intrinsic heterogeneity. Cellular senescence was robustly induced within 10 days upon exposure to 25 µg/mL bleomycin for 24 h. This resulted in increased β-galactosidase activity, enlarged cells, and elevated transcript levels of senescence-associated secretory phenotype (SASP) molecules (IL1b, TNFa, CCL2). The ability to contract upon a stimulus was reduced, as shown in live cell imaging studies. Reduced proliferation among others was indicated by a decreased abundance of proliferating cell nuclear antigen (PCNA), evident in a proteomic analysis, which also revealed massive changes (341 significantly increased and 372 decreased proteins). Bioinformatics analysis indicated a marked reduction in several cell-motility-associated proteins, whereas proteins linked to extracellular exosomes, the cytoskeleton, and lysosomal pathways were increased in abundance. To conclude, bleomycin treatment causes a rapid and robust induction of cellular senescence in a translational testicular cell model of peritubular cells. This model will enable future studies aiming to explore mechanisms and consequences of testicular aging.
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Registered trials
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