Evidence map›Paper›PMID 42173983›Full record

ArticleScientific reports2026

Sub-second extracellular impedance measurement of epithelial cell monolayers using step excitations and time-domain analysis.

Rongming Guo, Athena J Chien, Jake Hawks, Benjamin Magondu, Bo Yang, Xavier Acevedo, Adrienne L Watson, Bob Lewis, Chris Hatcher, Craig R Forest

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Rongming GuoG.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA. rguo61@gatech.edu.
Athena J ChienWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, USA.
Jake HawksSchool of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, USA.
Benjamin MagonduWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, USA.
Bo YangG.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA.
Xavier AcevedoWorld Precision Instruments, LLC., Sarasota, USA.
Adrienne L Watson *World Precision Instruments, LLC., Sarasota, USA.
Bob Lewis *World Precision Instruments, LLC., Sarasota, USA.
Chris Hatcher *World Precision Instruments, LLC., Sarasota, USA.
Craig R ForestG.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA.

Funding

Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
High-throughput robotic analysis of integrated neuronal phenotypesR01EY023173 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2012 to 2016
$4.3M
Automated cell-type-specific electrophysiology for understanding circuit dysregulation in Alzheimer's DiseaseRF1AG079269 · NIA · EMORY UNIVERSITY · PI FOREST, CRAIG, ROWAN, MATTHEW J.M. · 2022 to 2024
$2.4M
Scalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2M
NEI NIH HHS R01 EY023173NIA NIH HHS RF1 AG079269NIA NIH HHS RF1AG079269NIDA NIH HHS R01 DA029639NIDA NIH HHS R01DA029639NINDS NIH HHS R01 NS102727NINDS NIH HHS R01NS102727
6 · The paper itself

Abstract

Electrochemical impedance spectroscopy (EIS) is a versatile technique in epithelial biology, offering quantitative insights into barrier integrity, morphology, and apical-basolateral polarity non-invasively through measurements of transepithelial resistance (TER/TEER), capacitance (TEC), and membrane ratio (α). However, due to broad-spectrum frequency sweeps, EIS typically demands tens of seconds per measurement, limiting its applicability to faster biological phenomena. We present Time-domain Epithelial Impedance Measurement (TEIM), a method enabling sub-second extracellular impedance measurements of epithelial monolayers by combining step current excitations and time-domain voltage transient analysis while bypassing Fourier transforms. We experimentally demonstrate TEIM's ability to measure TER/TEER, TEC, α, and model-derived impedance spectrum every ∼0.3 s, which represents ∼100-fold time resolution improvement over EIS. TEIM's accuracy and precision were benchmarked against EIS using electrical circuits and human bronchial (16HBE) and colorectal (Caco-2) epithelial cell lines, yielding average errors for TER, TEC, and α ranging from 0.17-3.55%, 1.13-8.96%, and 0.59-26.35%, respectively. Applying TEIM to monitor Caco-2 responses to saponin revealed, for the first time, smoothly gated double-exponential transient TER and TEC dynamics too rapid to be adequately captured by EIS. Overall, TEIM offers a capable framework for probing rapid cellular electrophysiology, and offers a high-resolution alternative for dynamic assays in biological and pharmacological research.

Indexed as

Dielectric SpectroscopyEpithelial CellsCaco-2 CellsElectric ImpedanceHumans

Identifiers

PMID42173983
PMCPMC13408181

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.