Evidence map›Paper›PMID 42173011›Full record

ArticlePoultry science2026

Successful establishment of chicken primordial germ cell (PGC) lines from the endangered Slovakian indigenous chicken breed: the Oravka.

Jehan Nayga, Bence Lázár, Arnold Tóth, Mariann Molnár, Maria Teresa Salinas Aponte, Jaromír Vasícek, Andrej Balázi, Jakub Vozaf, Lucia Olexiková, Peter Chrenek and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jehan NaygaHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, Gödöllő, Hungary.
Bence LázárHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, Gödöllő, Hungary; National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Gödöllő, Hungary. Electronic address: lazar.bence@nbgk.hu.
Arnold TóthHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, Gödöllő, Hungary.
Mariann MolnárNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Gödöllő, Hungary.
Maria Teresa Salinas AponteHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, Gödöllő, Hungary.
Jaromír VasícekNPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia; Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Andrej BaláziNPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia.
Jakub VozafNPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia; Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Lucia OlexikováNPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia.
Peter ChrenekNPPC-Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia; Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Krisztina LiptóiNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Gödöllő, Hungary.
Elen GóczaHungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Animal Biotechnology Department, Gödöllő, Hungary.
Eszter VárkonyiNational Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Gödöllő, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PGCs offer a viable means for cryobanking both female and male avian genetic material. These early-migrating cells ultimately give rise to eggs and sperm cells, making them valuable for long-term conservation of poultry genetic resources. In this study, PGCs from the endangered Oravka chicken, a local breed native to Slovakia, were isolated, propagated in vitro, characterized, and cryopreserved. A total of 18 stable PGC lines were successfully established, comprising 12 female and 6 male lines. Characterization using immunohistochemistry, gene expression analysis, karyotyping, cell proliferation assays, TEM, and in vivo migration assays confirmed the identity and developmental competency of the in vitro cultured PGCs. A total of 108 cryopreserved samples were deposited into the gene bank. This study reports the first successful derivation, in vitro culture and cryobanking of stable PGC lines from the Oravka breed, providing a foundational resource for the conservation and future restoration of this distinctive local genetic lineage.

Indexed as

ChickensGerm CellsAnimalsCryopreservationEndangered SpeciesFemaleMaleSlovakiaGene bankIndigenousIn vitro cultureOravka chicken breedPrimordial germ cell

Identifiers

PMID42173011
PMCPMC13223939

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.