Vacuum-enhanced high-resolution 3D printing yields 11 200 valves and uniform 7 μm isoporous membranes.

High-resolution 3D printing holds promise for fabricating complex microfluidic devices, but challenges such as bubbles forming in the resin and becoming entrapped within printed layers, non-uniform illumination, and narrow processing windows limit yield, consistency, and scalability-particularly for dense, sub-10 μm features. Here we present a vacuum-enhanced 3D printing platform that integrates a custom printer, specialized resin formulation, and optimized protocols to overcome these barriers. We eliminate bubble formation and entrapment by applying a vacuum to remove dissolved gases from the resin and realize highly uniform illumination, with an irradiance standard deviation of just 0.18% across the full build area, while resolving downstream effects from the absence of dissolved oxygen. This approach dramatically expands reliable fabrication parameters and enables high-density integration without extensive manual optimization. We demonstrate the platform's capabilities by printing 11 200 membrane valves (150 μm diameter) in seven stacked layers of 1600 valves each, and by producing an array of 198 identical isoporous membranes with uniform 7 μm pores distributed across the full print area. These advances establish a pathway to scalable, high-density 3D-printed microfluidics.