Evidence map›Paper›PMID 42171359›Full record

ArticleMicrobiology spectrum2026

Dairy manure reception pits as reservoirs of extracellular DNA-associated antibiotic resistance genes.

Najmuj Sakib, Daniel Andersen, Laura Jarboe, Adina Howe

Abstract read
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Najmuj SakibDepartment of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA.ORCID 0000-0002-0875-6537
Daniel AndersenDepartment of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA.
Laura JarboeInterdepartmental Microbiology Graduate Program, Ames, Iowa, USA.ORCID 0000-0002-4294-4347
Adina HoweDepartment of Agricultural and Biosystems Engineering, Iowa State University, Ames, Iowa, USA.ORCID 0000-0002-7705-343X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dairy manure reception pits receive fresh manure and milkhouse wash water, and have short residence times, yet their role as reservoirs of extracellular antibiotic resistance genes (exARGs) remains poorly characterized. Here, we quantified six ARGs ( IMPORTANCE: To date, extracellular DNA (exDNA) has been shown to contribute to the spread of antibiotic resistance genes (ARGs) in the environment; however, few studies have evaluated its enrichment in short-term dairy pit-stored manure systems. This study demonstrates that dairy manure pits concentrate exDNA during short-term storage and serve as reservoirs for ARGs, along with mobile genetic elements that can facilitate subsequent gene transfer. The results of this study are a strong rationale for further investigation and targeted management strategies of exDNA in manure pits.

Indexed as

BacteriaDrug Resistance, BacterialDrug Resistance, MicrobialManureAnimalsAnti-Bacterial AgentsCattleDairyingDNA, BacterialExtrachromosomal DNAGenes, BacterialIntegronsAnti-Bacterial AgentsDNA, BacterialExtrachromosomal DNAManureantibioticsARGsdairy manureexDNAmanure pitMGEsresistance

Identifiers

PMID42171359
PMCPMC13340301

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.