ArticleCurrent research in food science2026
Elucidating the astringency perception mechanism of protocatechuic acid, a key astringent component in black rice, based on polyphenol-salivary protein interaction.
Article in Current research in food science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Black rice is rich in bran layer polyphenols, which enhance bioactivity but may reduce its consumer acceptability due to astringency. Polyphenol-salivary protein aggregation is the molecular basis of astringency. In this study, a saliva-induced precipitation approach with liquid chromatography-tandem mass spectrometry was used to identify protocatechuic acid (PA) as the key astringent component in black rice, accounting for 68% of total precipitated polyphenols. With α-amylase as a representative, the PA-salivary protein interaction was analyzed using fluorescence spectroscopy, isothermal titration calorimetry (ITC), and molecular docking. Fluorescence spectroscopy showed a quenching constant (
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