Evidence map›Paper›PMID 42168866›Full record

ArticleBMC plant biology2026

Long-term preservation strategy for legume root nodule phenotypes coupled with a comprehensive evaluation method.

Ya Zhao, Can Guo, Wen Zhang, Du Wang, Jun Jiang, Ao Liu, Wei Wang, Qin Han, Qi Zhang, Peiwu Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Ya Zhao *College of Engineering, China Agricultural University, Beijing, 100083, China.
Can Guo *Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Wen ZhangKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Du WangKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Jun JiangKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Ao LiuKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Wei WangCollege of Engineering, China Agricultural University, Beijing, 100083, China. playerwxw@cau.edu.cn.
Qin HanKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. hanqin@caas.cn.
Qi ZhangKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. zhangqi@caas.cn.
Peiwu LiKey Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.

Funding

Hubei Provincial Natural Science Foundation Youth Program JCZRQN202401143Major Project of Hubei Province Science & Technology 2023BBA002Major Science & Technolog Tasks of the Chinese Academy of Agricultural Sciences CAAS-ZDRW202416Special Projects of National Natural Sciences Foundation 32441047
6 · The paper itself

Abstract

Nodule color and morphology are key readouts of legume symbiotic performance. However, long-term preservation of post-excavation nodules with intact morphology, color, and microbial cleanliness remains a major challenge. This study developed a two-stage aqueous-phase preservation method (TAPP) that enables rapid structural fixation and long-term chemical stabilization. A comprehensive evaluation was subsequently established, incorporating composite morphological score (0-5), color difference (ΔE) and its piecewise slope over time ([Formula: see text]), and visible contamination grade (0-3). Peanut and soybean nodules from multiple regions and cultivars were tracked for 24 months under five preservation methods: TAPP, FormalinCu, TAPP-Resin, Resin, and AirDry. TAPP showed the best overall preservation, with composite morphological scores of 4.65 ± 0.14 for peanut and 4.63 ± 0.22 for soybean at 24 months, and no visible mold. Color change slowed over time: [Formula: see text] decreased from 1.83 to 1.10 ΔE·month

Indexed as

ArachisFabaceaeGlycine maxPreservation, BiologicalRoot Nodules, PlantPhenotypeSymbiosisTime FactorsColor-change kineticsComprehensive evaluationLegume nodulesPhenotypic morphologyTwo-stage aqueous-phase preservation technology (TAPP)

Identifiers

PMID42168866
PMCPMC13366862

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.