Evidence map›Paper›PMID 42168698›Full record

ArticleApplied microbiology and biotechnology2026

Quantitative proteomics identifies GntR as a novel potential DIVA antigen for bovine brucellosis.

Haoqing Lv, Xiaxia Liu, Yi Luo, Chunhui Ma, Lvfeng Yuan, Liyuan Liu, Wei Ran, Yinjuan Song, Suzi Zhang, Pengcheng Gao and 3 more

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Haoqing LvState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Xiaxia LiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Yi LuoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Chunhui MaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Lvfeng YuanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Liyuan LiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Wei RanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Yinjuan SongState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Suzi ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Pengcheng GaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Fuying ZhengState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Yuefeng ChuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. chuyuefeng@caas.cn.
Jian XuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. xujian@caas.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brucella is a zoonotic pathogen causing brucellosis. Current serological tests cannot differentiate infected from vaccinated animals (DIVA) due to antibody interference from live vaccines. This study used parallel accumulation-serial fragmentation (PASEF)-based quantitative proteomics to compare virulent S2308 and vaccine A19 strains of Brucella abortus. Among 2,131 identified proteins, 302 were differentially expressed, including 43 and 32 proteins exclusive to S2308 and A19, respectively. Bioinformatic analysis showed enrichment in metabolic and transport functions. The transcriptional regulator GntR family (GntR), with significantly high expression in S2308, was selected as a DIVA candidate antigen. Reverse transcription quantitative real-time PCR (RT-qPCR) and western blot confirmed significantly higher GntR transcription and expression in S2308 during logarithmic growth. Serological validation indicated stronger reactivity of GntR with sera from wild strain-infected cattle than from A19 vaccinated or negative cattle. An indirect enzyme-linked immunosorbent assay (iELISA) based on GntR was established and showed high sensitivity and specificity, consistent with standard bovine brucellosis diagnostics. Dynamic monitoring in a mouse model revealed that iELISA enabled DIVA differentiation from 0 to 21 days post-infection. Thus, GntR is a novel potential DIVA antigen, providing a robust serological tool to distinguish B. abortus wild strain infection from A19 vaccination in cattle. KEY POINTS: • PASEF-based quantitative proteomics to compare proteomic profiles of B. abortus S2308 and A19. • GntR transcription and expression are higher in Brucella S2308 than in A19 during logarithmic growth. • A novel potential DIVA antigen GntR was identified for bovine brucellosis.

Indexed as

Antigens, BacterialBacterial ProteinsBrucella abortusBrucellosis, BovineProteomicsTranscription FactorsAnimalsAntibodies, BacterialBrucella VaccineCattleEnzyme-Linked Immunosorbent AssayMiceSensitivity and SpecificityAntibodies, BacterialAntigens, BacterialBacterial ProteinsBrucella VaccineTranscription FactorsBrucella abortusDIVAGntRiELISAQuantitative proteomics

Identifiers

PMID42168698
PMCPMC13368942

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.