Evidence map›Paper›PMID 42167236›Full record

ArticleMolecular cell2026

Ribo-Tweezer: Rapid removal of ribosomal proteins reveals additional layers of post-transcriptional gene regulation.

Yuxiang Chen, Ching Pin Cheng, Kitra Cates, Georgi K Marinov, Travis C Lantz, Haojun Yang, Isabelle Liu, Naomi R Genuth, Christina Andronescu, Victoria Hung and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yuxiang ChenDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Ching Pin ChengDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Kitra CatesDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Georgi K MarinovDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Travis C LantzDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA; Department of Chemical and Systems Biology, Stanford School of Medicine, Stanford, CA, USA.
Haojun YangHelen Diller Family Comprehensive Cancer Center, UCSF, San Francisco, CA, USA; School of Medicine and Department of Urology, UCSF, San Francisco, CA, USA; Department of Cellular and Molecular Pharmacology, UCSF, San Francisco, CA, USA.
Isabelle LiuHelen Diller Family Comprehensive Cancer Center, UCSF, San Francisco, CA, USA; School of Medicine and Department of Urology, UCSF, San Francisco, CA, USA; Department of Cellular and Molecular Pharmacology, UCSF, San Francisco, CA, USA.
Naomi R GenuthDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Christina AndronescuDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA; Department of Human Biology, Stanford University, Stanford, CA, USA.
Victoria HungDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Abel BermudezDepartment of Radiology, Stanford School of Medicine, Stanford, CA, USA.
Daphna RothschildDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Joseph GeorgesonDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Sonia Bustos BarocioDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA.
Anshul KundajeDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA; Department of Computer Science, Stanford University, Stanford, CA, USA.
Sharon PitteriDepartment of Radiology, Stanford School of Medicine, Stanford, CA, USA.
Davide RuggeroHelen Diller Family Comprehensive Cancer Center, UCSF, San Francisco, CA, USA; School of Medicine and Department of Urology, UCSF, San Francisco, CA, USA; Department of Cellular and Molecular Pharmacology, UCSF, San Francisco, CA, USA.
Maria BarnaDepartment of Genetics, Stanford School of Medicine, Stanford, CA, USA. Electronic address: mbarna@stanford.edu.

Funding

Mechanisms of regulated translation control in cancer and its therapeutic implicationsR35CA242986 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI RUGGERO, DAVIDE · 2019 to 2025
$6.4M
Specialized Translational Control of Stem Cell Differentiation and Embryonic DevelopmentR01HD086634 · NICHD · STANFORD UNIVERSITY · PI BARNA, MARIA · 2016 to 2025
$5.5M
Predicting context-specific molecular and phenotypic effects of genetic variation through the lens of the cis-regulatory codeU01HG012069 · NHGRI · STANFORD UNIVERSITY · PI Anshul Kundaje · 2021 to 2026
$3.9M
Decoding the regulatory architecture of the human genome across cell types, individuals and diseaseU01HG009431 · NHGRI · STANFORD UNIVERSITY · PI PRITCHARD, JONATHAN K · 2017 to 2021
$3.5M
Fatty acid remodeling of the translatome during fasting and agingK99AG087723 · NIA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI YANG, HAOJUN · 2024 to 2025
$247k
NCI NIH HHS R35 CA242986NHGRI NIH HHS U01 HG009431NHGRI NIH HHS U01 HG012069NIA NIH HHS K99 AG087723NICHD NIH HHS R01 HD086634
6 · The paper itself

Abstract

The ribosome is a ribozyme, but it also acts as a dynamic regulator of gene expression. Although ribosomal protein (RP) composition varies, dissecting the functional contributions of individual RPs beyond their housekeeping roles is challenging because of the lack of tools for manipulation in situ. Here, we developed Ribo-Tweezer, a degron-based system directly tethered to mature ribosomes that enables rapid, reversible, and selective depletion of specific RPs. Using Ribo-Tweezer in mouse embryonic stem cells (mESC), we find a previously uncharacterized role for RACK1 in stem cell fate control via translational regulation of zinc-finger transcriptional networks and long interspersed nuclear element-1 (LINE1) expression. This translation-transcription coupling provides a mechanism by which translation control is further amplified in gene regulation. Distinct translational programs induced by RPLP0 and RPLP1 depletion further demonstrate RP-specific regulatory functions in translation. Together, these findings establish Ribo-Tweezer as a powerful platform that has illuminated selective functions for RPs in gene regulation, which gives biological meaning to ribosome heterogeneity.

Indexed as

Mouse Embryonic Stem CellsProtein BiosynthesisRibosomal ProteinsRibosomesRNA, CatalyticAnimalsDegronsGene Expression RegulationGTP-Binding ProteinsMiceNeoplasm ProteinsReceptors for Activated C KinaseTranscription, GeneticGTP-Binding ProteinsNeoplasm ProteinsReceptors for Activated C KinaseRibosomal ProteinsRNA, CatalyticLINE-1mESC differentiationPoly(A/U) leaderP-stalkRACK1ribosomeribosome heterogeneitytranslation control

Identifiers

PMID42167236
PMCPMC13286250

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.