Evidence map›Paper›PMID 42167121›Full record

ArticleEBioMedicine2026

HIV-1 virome profiling using HIV-PULSE to guide therapeutic and curative interventions.

Sofie De Braekeleer, Laurens Lambrechts, Liesbet Termote, Mareva Delporte, Kavita Mehta, Vasiliki Matzaraki, Evy E Blomme, Philippe Lemey, Bram Vrancken, Casper Rokx and 6 more

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Article in EBioMedicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

16 authors.

Sofie De BraekeleerHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Laurens LambrechtsHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Liesbet TermoteHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Mareva DelporteHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Kavita MehtaHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Vasiliki MatzarakiDepartment of Internal Medicine and Infectious Diseases, Radboudumc, Nijmegen, Netherlands.
Evy E BlommeHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Philippe LemeyDepartment of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Evolutionary and Computational Virology, KU Leuven, Leuven, Belgium.
Bram VranckenDepartment of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Evolutionary and Computational Virology, KU Leuven, Leuven, Belgium.
Casper RokxDepartment of Internal Medicine and Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, Netherlands.
Janneke StalenhoefDepartment of Internal Medicine and Infectious Diseases, OLVG, Amsterdam, Netherlands.
Marvin A H BerrevoetsDepartment of Internal Medicine and Infectious Diseases, Elizabeth-Tweesteden Ziekenhuis, Tilburg, Netherlands.
Andre J van der VenDepartment of Internal Medicine and Infectious Diseases, Radboudumc, Nijmegen, Netherlands.
Sarah GerloHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
Sofie RutsaertHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Linos VandekerckhoveHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium; University Hospital Ghent, Ghent, Belgium. Electronic address: Linos.Vandekerckhove@UGent.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe persistent HIV-1 reservoir remains a major barrier to curing HIV-1, underscoring the need for a detailed understanding of its composition to inform targeted interventions.

methodsHere, we apply the HIV Proviral Unique molecular identifier-mediated Long-read Sequencing (HIV-PULSE) assay to characterise the viral reservoir in 90 individuals on suppressive antiretroviral therapy. By generating near full-length proviral sequences, HIV-PULSE enables multiple layers of in silico analyses across the HIV-1 genome.

findingsThese data support the construction of participant-specific HIV virome profiles, integrating assessments of proviral intactness, tropism, genetic diversity, archived drug resistance mutations and susceptibility to selected immune-based therapies (broadly neutralising antibodies and HIV-specific immune-mobilising monoclonal T cell receptor therapy IMC-M113V).

interpretationOur findings demonstrate that HIV-PULSE provides a comprehensive platform for individualised reservoir profiling, advancing future treatment perspectives and efforts towards an HIV-1 cure.

fundingThis research is part of the 2000HIV study, supported by ViiV Healthcare.

Indexed as

HIV-1HIV InfectionsAnti-HIV AgentsDrug Resistance, ViralGenetic VariationGenome, ViralHigh-Throughput Nucleotide SequencingHumansMutationProvirusesViral LoadViral TropismAnti-HIV AgentsDrug resistanceHIVLong-read sequencingTherapy screeningViral reservoir

Identifiers

PMID42167121
PMCPMC13199670

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.