ArticleSTAR protocols2026
Protocol for isolating cells from a single cryopreserved gastrointestinal endoscopic biopsy for single-cell RNA sequencing.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Techniques exist to isolate cells from fresh intestinal tissue; however, approaches for recovering viable cells from cryopreserved duodenal and colonic specimens are lacking. Here, we present a protocol for isolating cells from a single cryopreserved gastrointestinal endoscopic biopsy for single-cell RNA sequencing (scRNA-seq). We describe steps for thawing and washing cryopreserved endoscopic biopsies, enzymatic digestion of biopsies, and purification preparation. We then detail procedures for dead cell removal, quantification of cell recovery, and 10X Gel Bead-in-Emulsion (GEM) generation and library preparation. For complete details on the use and execution of this protocol, please refer to Richards et al.
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