Evidence map›Paper›PMID 42165938›Full record

ArticleJournal of assisted reproduction and genetics2026

One-step warming procedure improves embryonic development of vitrified mouse oocytes by mitigating mitochondrial damage.

Hao Wang, Mingzhe Li, Qi Jiang, Qiaodan Li, Xue Sun, Youjiang Li, Xiao Chen, Jian Xu, Ri-Cheng Chian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hao WangDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Mingzhe LiLaboratory Animal Center, Qilu Hospital of Shandong University, Jinan, China.
Qi JiangCenter for RNA Medicine, International Medical School, International Institutes of Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, China.
Qiaodan LiDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Xue SunDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Youjiang LiDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China.
Xiao ChenDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China. cxiao@zju.edu.cn.
Jian XuDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, China. xuj@zju.edu.cn.
Ri-Cheng ChianCenter for Reproductive Medicine, Heilongjiang Provincial Hospital, Harbin, China. rchian@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate whether the one-step warming (OW) procedure affects oocyte survival and developmental potential after fertilization in vitrified oocytes.

methodsStandard vitrified mouse oocytes were warmed using two different procedures: (1) standard multi-step warming (SW) and (2) one-step warming (OW). The survival rates and early embryonic development potentials of the warmed oocytes were compared following insemination by piezo-ICSI. Spindle integrity of the warmed oocytes was evaluated by α-tubulin/Hoechst staining, and mitochondrial status was assessed using Mitotracker/JC-1 staining, with mitochondrial damage analyzed by Confocal microscope. Additionally, the apoptosis status of the resulting blastocysts in each group was compared using a TUNEL assay.

resultsThe OW procedure maintained oocyte survival and fertilization rates comparable to those of the SW procedure, but significantly (P < 0.05) increased blastocyst development after fertilization. Mechanistic measures revealed that the OW procedure improved embryonic developmental potential by mitigating mitochondrial damage compared with the SW procedure.

conclusionsThese findings provide a theoretical foundation for optimizing the OW procedure for already vitrified and stored oocytes in ARTs.

Indexed as

CryopreservationEmbryonic DevelopmentMitochondriaOocytesVitrificationAnimalsApoptosisBlastocystFemaleFertilization in VitroMiceSperm Injections, IntracytoplasmicEmbryosMitochondrial damageMouseOne-step warmingOocytes

Identifiers

PMID42165938
PMCPMC13518644

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