Evidence map›Paper›PMID 42165845›Full record

ArticlePlanta2026

Optimization of Agrobacterium-mediated transformation of commercial heirloom tomato cultivars to develop novel traits via CRISPR/Cas9 genome editing.

Jordan Oxendine, Elsa Ibarra-Reyes, Junchi Ma, Carrie Li, Sarah Baron, Allison E Hwang, Ruiting Wang, Daniel Rodriguez-Leal

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jordan Oxendine *Tomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Elsa Ibarra-Reyes *Tomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Junchi MaTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Carrie LiTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Sarah BaronTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Allison E HwangTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Ruiting WangTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA.
Daniel Rodriguez-LealTomato Lab, Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA. danielrl@umd.edu.ORCID http://orcid.org/0000-0003-1871-7432

Funding

California Tomato Research Institute 89365University of Maryland MD-PSLA-243084University of Maryland Startup funds
6 · The paper itself

Abstract

MAIN

conclusionTrait development for commercial heirloom tomatoes can be advanced by optimization of tissue culture and transformation via Agrobacterium and CRISPR/Cas9 mutagenesis. Genetic improvement using new genome editing approaches relies on the efficient delivery of the CRISPR/Cas system in the vegetable crop tomato. Previous protocols for tomato transformation have primarily focused on a handful of cultivars (M82, Ailsa Craig, Microtom, Sweet-100) with very little commercial relevance, and it is not clear if these protocols can be implemented directly in other commercially relevant varieties. Heirloom tomatoes are sought for their deep and diverse flavor but have not been subjected to systematic crop improvement via conventional breeding or biotechnology approaches such as transgenesis or genome editing. Therefore, we tested the transformation and regeneration capacity of six different heirloom cultivars known for their superior taste and market relevance in the US. Subsequently, we optimized rooting conditions and used the GRF4-GIF1 chimeric developmental regulator to successfully recover transgenic plants. Finally, we evaluated the efficiency of targeted genetic modification using the CRISPR/Cas9 genome editing system in several of these cultivars. We demonstrate that our optimizations led to successful transformation of several heirloom varieties, including the generation of edited plants for target genes modifying plant architecture and flowering time. Our results set the foundation for a biotechnology platform to deliver improved traits to local and regional heirloom varieties using genome editing.

Indexed as

AgrobacteriumCRISPR-Cas SystemsGene EditingSolanum lycopersicumPlant BreedingPlants, Genetically ModifiedTransformation, GeneticBiotechnologyGeneticsTissue cultureTrait development

Identifiers

PMID42165845
PMCPMC13194190

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.