ArticleTranslational vision science & technology2026
Evaluating the Influence of the Lens Autofluorescence on Adaptive Optics Fluorescence Lifetime Imaging Ophthalmoscopy.
Article in Translational vision science & technology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
- Update of
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: To assess the impact of lens autofluorescence on adaptive optics fluorescence lifetime imaging ophthalmoscopy (AOFLIO). Methods: Eighteen subjects aged 23 to 68 years with normal chorioretinal health and phakic lenses were imaged using a research-grade AOFLIO instrument. Retinal autofluorescence was excited with a pulsed laser with wavelength at 473 nm and detected in two spectral channels (500-560 nm and 560-720 nm). AOFLIO images were acquired from the foveal center to 10° nasal retina. Autofluorescence decay was modeled using bi-exponential and tri-exponential functions, with and without accounting for the early arrival of lens autofluorescence. The contribution of lens signal was evaluated in relation to age and retinal location. Results: The tri-exponential model that accounted for the early arrival of lens autofluorescence demonstrated superior agreement with measured decays. The amplitude coefficient of lens contribution was 3% to 4%. However, this component accounted for 19.4% to 29.0% of photon-weighted signals and resulted in a 22.8% to 42.9% overestimation of the mean retinal lifetime when uncorrected. The lens effect was more pronounced in the short spectral channel. The time shift parameter of lens signal was associated with age (P = 0.023) and correlated with axial length (P = 0.036). Conclusions: AOFLIO substantially suppresses lens autofluorescence; however, residual lens signal persists and can disproportionately bias retinal autofluorescence lifetime estimates. Accurate correction of lens contributions is essential for reliable AOFLIO measurements. Translational Relevance: Minimizing the impact of lens autofluorescence in AOFLIO ensures accurate assessments of metabolic status in the retina and retinal pigment epithelium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.