Evidence map›Paper›PMID 42165602›Full record

ArticleMicrobiology spectrum2026

Development of an indirect ELISA based on a multi-epitope recombinant protein for antibody detection against African swine fever virus.

Lixinjie Liu, Qichao Chen, Linmeng Yu, Wei Wu, Shuang Mou, Huihui Guo, Chen Tan, Qingyun Liu, Dang Wang, Huanchun Chen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lixinjie Liu *National Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Qichao Chen *National Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Linmeng YuNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Wei WuNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Shuang MouNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Huihui GuoNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Chen TanNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.ORCID 0000-0003-4622-4483
Qingyun LiuNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Dang WangNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Huanchun ChenNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.
Xiangru WangNational Key Laboratory of Agricultural Microbiology, College of Veterinary, Huazhong Agricultural University, Wuhan, Hubei, China.ORCID 0000-0003-0191-1378

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever (ASF) is a highly contagious and fatal hemorrhagic disease of swine. While vaccines have been licensed in Vietnam and the Philippines, their global application remains constrained by insufficient large-scale validation of safety and efficacy, rendering rapid and reliable diagnostic assays crucial for effective prevention and control. In this study, a multi-epitope antigen (MEA) was rationally designed by utilizing informatics to predict and select 15 conserved B-cell epitopes from four major African swine fever virus (ASFV) structural proteins: p30, p54, p72, and pA104R. The recombinant MEA was expressed in

Indexed as

African Swine FeverAfrican Swine Fever VirusAntibodies, ViralEpitopes, B-LymphocyteAnimalsAntigens, ViralEnzyme-Linked Immunosorbent AssayEscherichia coliRecombinant ProteinsReproducibility of ResultsSensitivity and SpecificitySwineVietnamViral Structural ProteinsAntibodies, ViralAntigens, ViralEpitopes, B-LymphocyteRecombinant ProteinsViral Structural ProteinsAfrican swine fever virusindirect ELISAmulti-epitope antigen

Identifiers

PMID42165602
PMCPMC13340067

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.