Evidence map›Paper›PMID 42165131›Full record

ArticleNucleic acids research2026

Regulation of cyclic lipopeptide orfamide A biosynthesis in Pseudomonas protegens by the Gac-Rsm-LuxR Cascade.

Ruoyi Wang, Chenjie Yu, Yan Song, Lulu Zhang, PingPing Li, Linli Zhu, Yali Fu, Rongshi Chen, Guohua Ding, Rainer Borriss and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

14 authors.

Ruoyi WangThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Chenjie YuThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Yan SongThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Lulu ZhangThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
PingPing LiThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Linli ZhuThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Yali FuThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Rongshi ChenThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Guohua DingModern Agricultural Analysis and Testing Center, Nanjing Agricultural University, 211800 Nanjing, China.
Rainer BorrissInstitute of Biology, Humboldt University, 10115 Berlin, Germany.
Xuewen GaoThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Junqing QiaoInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 210014 Nanjing, China.
Qin GuThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.
Huijun WuThe Sanya Institute of Nanjing Agricultural University, Nanjing Agricultural University, 572024 Sanya, China.ORCID 0000-0001-5586-1607

Funding

Basic Research Program of Jiangsu BK20250381Joint Research Program of State Key Laboratory of Agricultural and Forestry Biosecurity SKLJRP2510Ministry of Education of China JYB2025XDXM703Mongolia Autonomous Region Key R&D and Achievement Transformation Project 2023YFDZ0011National Natural Science Foundation of China 32472643National Natural Science Foundation of China 32502571
6 · The paper itself

Abstract

Orfamide A, a lipopeptide produced by Pseudomonas protegens Pf-5, is a key determinant of its biocontrol properties. In this study, we investigated the regulatory interactions among the GacS/A two-component system, small RNAs (sRNAs), repressor proteins, and two LuxR-type transcription factors in orfamide A biosynthesis. We found that GacS/A indirectly regulates orfamide A production by enhancing transcription of three sRNAs (RsmX, RsmY, and RsmZ). RsmY and RsmZ synergistically relieve repression by RsmA and RsmE, while RsmX plays a lesser role, likely counteracting only one repressor. LuxR-type transcription factors, LuxR1 and LuxR2, which positively regulate orfamide A synthesis, are directly repressed by RsmA and RsmE via binding to their 5' untranslated regions, linking them to the Gac-Rsm signaling cascade. We further demonstrated that LuxR2 activates luxR1 expression, which in turn facilitates orfamide A production by binding to the promoter of the orfamide A biosynthetic gene cluster. Importantly, we showed that this entire regulatory cascade operates in the rhizosphere and directly influence biocontrol efficacy. These findings provide a comprehensive understanding of the Gac-Rsm-LuxR pathway in orfamide A biosynthesis and offer valuable insights for the development of biocontrol agents based on Pseudomonas strains.

Indexed as

Bacterial ProteinsGene Expression Regulation, BacterialLipopeptidesPeptides, CyclicPseudomonasRepressor ProteinsTrans-ActivatorsPromoter Regions, GeneticRNA, Small UntranslatedTranscription FactorsBacterial ProteinsLipopeptidesLuxR autoinducer binding proteinsPeptides, CyclicRepressor ProteinsRNA, Small UntranslatedTrans-ActivatorsTranscription Factors

Identifiers

PMID42165131
PMCPMC13187841

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.