ArticleFrontiers in cellular and infection microbiology2026
Rapid identification of
Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Introduction: Many edible fungi and poisonous mushrooms share similar morphologic characteristics, making them difficult to distinguish with the naked eye. Lepiota brunneoincarnata is a major lethal toxic mushroom widely distributed in China and responsible for frequent poisoning incidents across the country. Methods: The present study developed a loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the translation elongation factor 1-a (tef1) sequence of L. Brunneoincarnata. Results: A set of specific LAMP primers were designed for the specific detection of L. brunneoincarnata, and the assay showed no amplification from 63 non-target fungal specimens. In this experiment, the limit of detection for DNA concentration was 100 pg/mL, and the limit of detection for spore concentration was 10 spores/mL. The method also successfully detected L. brunneoincarnata in boiled and digested mixed mushroom samples, and the whole detection procedure can be completed within 60 minutes at 63 °C. Discussion: The study is suitable for rapid identification of L. brunneoincarnata in China, and the method has potential for further optimization toward field application.
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