Role of Caspase-8 on Retinal Endothelial Cell Migration and Angiogenesis.

Paola Campano, Laura Valdez, Lili Guerra, Benxu Cheng, Andrew Tsin

Abstract read

In one paragraph

Article in Annals of vascular medicine and research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics.Journal of proteome research · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Paola CampanoDepartment of Molecular Science, The University of Texas Rio Grande Valley - School of Medicine, USA.

Laura ValdezDepartment of Molecular Science, The University of Texas Rio Grande Valley - School of Medicine, USA.

Lili GuerraDepartment of Molecular Science, The University of Texas Rio Grande Valley - School of Medicine, USA.

Benxu ChengDepartment of Molecular Science, The University of Texas Rio Grande Valley - School of Medicine, USA.

Andrew TsinDepartment of Molecular Science, The University of Texas Rio Grande Valley - School of Medicine, USA.

Funding

Cell-Meditated Inflammatory Pathway and Diabetic RetinopathyR15EY033551 · NEI · UNIVERSITY OF TEXAS RIO GRANDE VALLEY · PI TSIN, ANDREW T C · 2022 to 2023

$624k

NEI NIH HHS R15 EY033551

6 · The paper itself

Abstract

Pathological angiogenesis is the hallmark of proliferative diabetic retinopathy (DR). In response to prolonged tissue injury, human retinal microvascular endothelial (HRMEC) cells form new blood vessels to ensure the supply of oxygen and nutrients; however, progressive fibrovascular proliferation can eventually lead to angiogenesis, retinal detachment, and blindness. Caspase-8, a responsible protease for apoptosis has also been shown to modulate pathological and developmental angiogenesis but whether it exerts this role in a hyperglycemic environment has not yet been explored. Here we demonstrate that high glucose decreased HRMEC cell viability and increased VEGF secretion. Pro-Caspase 8 expression was measured by RT-PCR and caspase 8 enzyme activity was confirmed by Caspase-Glo assay. In addition, Caspase-8 KD resulted in an inhibition of cell migration, and a significant reduction of p38 phosphorylation. Taken together, our data suggest that Caspase-8 may play a role in angiogenesis by promoting HRMEC migration via p38 MAPK phosphorylation.

Indexed as

AngiogenesisCaspase-8Cell migrationp38Retinal endothelial cells

Identifiers

PMID42164054

PMCPMC13186181

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.