A standardised and reproducible method for

Background: The rapidly changing landscape of abdominal transplantation including regional normothermic perfusion of abdominal organs during procurement, broadening indications for multi-visceral transplantation (MVT) in both benign and malignant pathology and the ever increasing gap between organ supply and demand have necessitated the normalisation of expanded criteria donors (ECD). Key to mitigating the increased risk of delayed or non-function of ECD organs is the development of suitable preclinical animal models that allow for the development of reliable viability assessment as well as reconditioning and repair protocols that can thereafter be translated to humans. Due to physiological and anatomical similarities to humans, pigs make ideal pre-clinical animal models. Our group has recently concluded a study assessing the feasibility of machine perfusion and reanimation in a porcine model. In this paper, the authors describe a reproducible Methods: Twenty-six adult female Large White pigs ( Results: We describe the stepwise surgical technique of multi-organ retrieval as a combined organ block. The average procedure time was 108.3 (range, 57-179) min. Conclusions: This surgical technique facilitated retrieval of all twenty-six organ blocks for experiments in MVT. The warm ischemia duration is minimal and closely replicates donation following brain death (DBD) in humans. With minor modifications, a donation following circulatory death (DCD) model would also be achievable. This technique is reproducible and safe and can be used to retrieve abdominal organs collectively for