Evidence map›Paper›PMID 42163840›Full record

ArticleJournal of the American Chemical Society2026

Directed Evolution of Threonine Aldolases for the Photobiocatalytic Synthesis of β-Branched-α-Tetrasubstituted and Other Noncanonical Amino Acids.

Tian-Ci Wang, Xiangyu Zhang, Zheng Zhang, Guodong Rao, Zhiyu Bo, Pei-Pei Xie, Jiedong Li, Qiang Yang, Xiaoyong Li, R David Britt and 3 more

Abstract read
In one paragraph

Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Tian-Ci WangDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.
Xiangyu ZhangDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Zheng ZhangDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.ORCID 0009-0001-8554-3076
Guodong RaoDepartment of Chemistry, University of California Davis, Davis, California 95616, United States.ORCID 0000-0001-8043-3436
Zhiyu BoDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.ORCID 0009-0002-1463-2829
Pei-Pei XieDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.ORCID 0000-0002-7801-5232
Jiedong LiDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.
Qiang YangSynthetic Molecule Design and Development, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, United States.ORCID 0000-0003-3762-5015
Xiaoyong LiSynthetic Molecule Design and Development, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, United States.ORCID 0000-0002-3518-3746
R David BrittDepartment of Chemistry, University of California Davis, Davis, California 95616, United States.ORCID 0000-0003-0889-8436
Jiang WangSynthetic Molecule Design and Development, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, United States.ORCID 0000-0003-2357-8246
Peng LiuDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.ORCID 0000-0002-8188-632X
Yang YangDepartment of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California 93106, United States.ORCID 0000-0002-4956-2034

Funding

Supplement:Spectroscopy Investigations of Metalloenzyme MechanismsR35GM126961 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI R. David Britt · 2018 to 2026
$3.7M
Pyridoxal Radical Biocatalysis for the Stereoselective Production of Non-Canonical Amino AcidsR01EB036084 · NIBIB · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Yang Yang · 2025 to 2026
$1.0M
NIBIB NIH HHS R01 EB036084NIGMS NIH HHS R35 GM126961
6 · The paper itself

Abstract

The convergent, stereoselective, and protecting-group-free synthesis of noncanonical amino acids, particularly those bearing a tetrasubstituted α-stereogenic center, remains a challenging task. By leveraging pyridoxal radical biocatalysis, we developed photobiocatalytic oxidative coupling methods for the assembly of α-tetrasubstituted amino acids, including β-branched variants, in a diastereo- and enantioselective fashion. Through repurposing and directed evolution of

Indexed as

Amino AcidsDirected Molecular EvolutionGlycine HydroxymethyltransferaseBiocatalysisPhotochemical ProcessesStereoisomerismThermotoga maritimaAmino AcidsGlycine Hydroxymethyltransferase

Identifiers

PMID42163840
PMCPMC13679966

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.