Evidence map›Paper›PMID 42162553›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Characterization of Adipocyte Precursor Cells (APCs) by Lineage Tracing, Flow Cytometry, and Cytometry Time of Flight (CyTOF).

Qiwei Zhang, Nathaniel Vo, Ju Hee Lee, Azadeh Yeganeh, Kafi Ealey, Hoon-Ki Sung

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qiwei ZhangTranslational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Nathaniel VoTranslational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Ju Hee LeeTranslational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Azadeh YeganehTranslational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Kafi EaleySchool of Nutrition, Toronto Metropolitan University, Toronto, ON, Canada. kealey@torontomu.ca.
Hoon-Ki SungTranslational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada. hoon-ki.sung@sickkids.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immuno-phenotyping, quantification, and isolation of distinct populations, which critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of APCs using flow cytometry, FACS, and cytometry by time-of-flight (CyTOF), defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the cellular origin and plasticity of APCs.

Indexed as

AdipocytesCell LineageFlow CytometryStem CellsAdipose TissueAnimalsCell DifferentiationCell SeparationMiceMice, TransgenicAdipocyte precursor cells (APCs)Adipose biologyCre-recombinase transgenic mouse modelCyTOFFlow cytometryLineage tracing

Identifiers

PMID42162553

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.