Gene Expression Analysis on Single Myofibers from Mouse Edl and Soleus Muscles.

Amairani Cancino-Bello, Mauricio Hernández-Somilleda, Estela G García-González, J Manuel Hernández-Hernández

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Amairani Cancino-BelloLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.

Mauricio Hernández-SomilledaLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.

Estela G García-GonzálezLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.

J Manuel Hernández-HernándezLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360. jose.hernandezh@cinvestav.mx.

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6 · The paper itself

Abstract

Skeletal muscle is composed of different myofiber types with specific transcriptomic, proteomic, and metabolic identities. As such, skeletal muscle represents a valuable model to study cell differentiation and gene expression regulation. However, many molecular analyses of skeletal muscle are performed on whole muscle, with the caveat that many different cell types that constitute this organ contribute to the experimental results. Here, we describe a method to efficiently isolate individual myofibers from two muscle types and provide a guide to perform quantitative gene expression analysis of representative myosin heavy chain genes.

Indexed as

Gene Expression ProfilingMuscle Fibers, SkeletalMuscle, SkeletalAnimalsMiceMyosin Heavy ChainsSingle-Cell Gene Expression AnalysisMyosin Heavy ChainsEene expressionExtensor digitorum longusMyh4Myh7Myofibers isolationqPCRSkeletal muscleSoleus

Identifiers

PMID42162552

DOI10.1006/meth.2001.1262

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