Evidence map›Paper›PMID 42162551›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

MicroRNA Quantification on Fast and Slow-Twitch Skeletal Muscles of Mouse.

Magally Ramírez-Ramírez, María Luisa Benítez-Hess, Estela G García-González, J Manuel Hernández-Hernández

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Magally Ramírez-RamírezLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.
María Luisa Benítez-HessLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.
Estela G García-GonzálezLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360.
J Manuel Hernández-HernándezLaboratorio de Epigenética de la Regeneración Muscular, Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México, México, 07360. jose.hernandezh@cinvestav.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

microRNAs (miRNAs) are noncoding RNAs of approximately 24 nucleotides long that are expressed in multiple tissues and organs. miRNAs regulate the expression of specific target genes, and their expression profile can provide insight into the cell biology altered by diseases or drug treatments. Research has demonstrated that several miRNAs regulate muscle differentiation and the specification of fiber type. Hence, an established tool to routinely quantify miRNA abundance is of the utmost importance. The stem-loop quantitative reverse transcription PCR (RT-qPCR) is a methodology that allows the analysis of a specific miRNA with high sensitivity. Upon hybridization with a structured stem-loop oligonucleotide to the 3' end of the target miRNA, a retro-transcription is possible for further qPCR quantification. In this chapter, we describe a protocol to quantify miRNAs in dissected mouse fast and slow-twitch muscle.

Indexed as

MicroRNAsMuscle Fibers, Fast-TwitchMuscle Fibers, Slow-TwitchAnimalsMiceReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionMicroRNAsFast-twitch fiberMicroRNAmiRNAs isolationRNA isolationSkeletal muscleSlow-twitch fiberStem–loop PCR

Identifiers

PMID42162551

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.