Evidence map›Paper›PMID 42162547›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Preparation of Mouse Embryonic Eye Sections for Routine Histology.

Esmeralda Lira-Romero, Francisco Javier Estrada-Mena, Israel Ramírez-Sánchez

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Esmeralda Lira-RomeroLaboratorio de Biología Molecular, Escuela de Medicina, Universidad Panamericana, Donatello 59, México City, 03920, México. elira@up.edu.mx.
Francisco Javier Estrada-MenaLaboratorio de Biología Molecular, Escuela de Medicina, Universidad Panamericana, Donatello 59, México City, 03920, México.
Israel Ramírez-SánchezSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, 11340, México. iramirezs@ipn.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eye development is a complex process that begins during the first stages of embryonic development; during its formation, a series of cell proliferation, migration, and differentiation events occur. A frequently used laboratory method is tissue staining with two dyes known as hematoxylin-eosin (H&E). H&E staining allows us to observe the arrangement of the cell layers and the formation of characteristic structures. It is fundamental for research in developmental biology since it facilitates a comprehensive analysis of the formation of organs and systems.In this chapter, we will describe the procedure for acquiring H&E-stained images of the embryonic eye structure, specifically focusing on the retina. This technique serves as a fundamental tool for understanding ocular morphogenesis, organogenesis, and structural modifications resulting from experimental interventions.

Indexed as

Embryo, MammalianEyeRetinaAnimalsEosine Yellowish-(YS)HematoxylinMiceOrganogenesisStaining and LabelingEosine Yellowish-(YS)HematoxylinDescalingHematoxylin–eosin stainHistological sectionsMouse embryonic eyeParaffinPerfusionRetina

Identifiers

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.