ArticleCardiovascular engineering and technology2026
Impact of Tissue Freezing on the Functional and Biomechanical Characteristics of Porcine Aortic Roots.
Article in Cardiovascular engineering and technology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
purposeFreeze-preservation of porcine aortic tissue is often necessary for experimental planning and standardization, but the impact of freezing on aortic root properties has not been systematically investigated. This study evaluated the effects of freezing and thawing on the biomechanical, fluid dynamic, and ultrasonographic characteristics of porcine aortic roots.
methodsA total of 32 porcine hearts were obtained from a local abattoir. Specimens were randomized to a fresh group (immediate testing) or a thawed group (storage at - 20 °C for 30 days followed by thawing in saline). Uniaxial tensile testing was performed on samples from the sinotubular junction (STJ) and non-coronary cusp (NCC) (n = 10 per group). Functional testing was conducted in a pulsatile flow loop (n = 6 per group), with mean aortic pressure gradient, forward and retrograde flow measured and the regurgitant fraction calculated. Ultrasonography was used to assess annular diameters and coaptation lengths.
resultsTensile testing revealed no statistically significant differences in E-module, maximum stress, maximum load, or stiffness between fresh and thawed specimens at either the STJ or NCC. Mean aortic gradient, retrograde flow and regurgitant fraction did not differ. Ultrasonography showed no statistically significant changes in annular geometry or leaflet coaptation length after freezing and thawing.
conclusionFreezing and thawing did not result in measurable differences in the biomechanical, fluid dynamic, or geometrical properties of porcine aortic roots under the tested protocol and within the sensitivity of our measurements. These findings support the use of thawed porcine aortic roots in experimental cardiovascular research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.