Evidence map›Paper›PMID 42161906›Full record

ArticleNature communications2026

N-glycopedia: libraries for native N-glycan structural analysis.

Christopher Ashwood, Richard D Cummings

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

  • Update of
    2025
5 · Who and what money

Authors and funding

2 authors.

Christopher AshwoodDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0001-5944-6179
Richard D CummingsDepartment of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. rcummin1@bidmc.harvard.edu.ORCID http://orcid.org/0000-0002-8918-5034

Funding

Protein-Glycan Interaction Resource at the National Center for Functional Glycomics (NCFG)R24GM137763 · NIGMS · BETH ISRAEL DEACONESS MEDICAL CENTER · PI RICHARD D CUMMINGS · 2020 to 2026
$5.8M
Glycoproteomics and the Glycosylation Code of the Brain in Asymptomatic and Symptomatic Alzheimer’s DiseaseRF1AG062181 · NIA · BETH ISRAEL DEACONESS MEDICAL CENTER · PI CUMMINGS, RICHARD D, SEYFRIED, NICHOLAS THOMAS · 2018 to 2018
$4.1M
Engineering Escherichia coli for glycosylation of complex human proteinsR44GM088905 · NIGMS · GLYCOBIA, INC. · PI FISHER, ADAM CHARLES · 2011 to 2012
$1.6M
A Comprehensive 5K plus Glycan MicroarrayR43GM123820 · NIGMS · Z BIOTECH, LLC · PI ZHANG, JIAN · 2017 to 2018
$305k
Humanizing N-linked glycosylation in Escherichia coliR43GM088905 · NIGMS · GLYCOBIA, INC. · PI FISHER, ADAM CHARLES · 2009 to 2009
$116k
NIA NIH HHS RF1 AG062181NIGMS NIH HHS R24 GM137763NIGMS NIH HHS R43 GM088905NIGMS NIH HHS R43 GM123820NIGMS NIH HHS R44 GM088905NIH HHS HHSN272201400008CU.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R24GM137763U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging) R01AG062181
6 · The paper itself

Abstract

Measurements of glycans modifying glycoproteins are hampered by the lack of standards that reflect the wide diversity in structure typically observed. To this end we exploit a large library of N-glycan standards comprised of a unique collection of 226 N-glycans including oligomannose, hybrid, and complex-type and apply a method employing porous graphitised carbon (PGC) and liquid chromatography mass spectrometry (PGC-LC-MS) to provide a high-resolution separation and characterisation of underivatized N-glycan structures. Chromatogram libraries arising from this study include retention time data, diagnostic fragments, and validated structural assignments, providing a robust platform for both targeted and discovery-based glycomics. Here we establish this generated data as an N-glycopedia, the resource in which researchers can compare this collective data to N-glycans under study and overcome the limitations of only having compositional data and predicted structures. The technology is easily expandable to include additional N-glycans as new standards become available.

Indexed as

GlycomicsGlycoproteinsPolysaccharidesGlycosylationLiquid Chromatography-Mass SpectrometryGlycoproteinsPolysaccharides

Identifiers

PMID42161906
PMCPMC13381884

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.