Evidence map›Paper›PMID 42161268›Full record

ArticleCell reports methods2026

Development of a human iPSC-derived corticospinal tract-on-a-chip.

Andriana Charalampopoulou, Arens Taga, Khalil Rust, Evelyn Luciani, Katherine Marshall, Elliot Montgomery, Anuradha Mansinghka, Richa Singh, Yang Zhao, Christine O'Keefe and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Andriana CharalampopoulouDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Arens TagaDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Khalil RustDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Evelyn LucianiDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Katherine MarshallDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Elliot MontgomeryDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Anuradha MansinghkaDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Richa SinghDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Yang ZhaoDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Christine O'KeefeDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Tza-Huei WangDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Arun VenkatesanDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Christa Whelan HabelaDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA.
Nicholas John MaragakisDepartment of Neurology, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: nmaragak@jhmi.edu.

Funding

Research Education Program for Residents and Fellows in Neurology - PorrasR25NS065729 · NINDS · JOHNS HOPKINS UNIVERSITY · PI HILLIS, ARGYE E. · 2009 to 2024
$3.3M
Modulating an Astrocyte Hemichannel to Delay Spatial and Temporal Progression in ALS.R01NS117604 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MARAGAKIS, NICHOLAS J · 2020 to 2024
$1.9M
Modeling genetic risk for epilepsy using IPSC and animal models.K08NS102526 · NINDS · JOHNS HOPKINS UNIVERSITY · PI HABELA, CHRISTA · 2020 to 2024
$996k
NINDS NIH HHS K08 NS102526NINDS NIH HHS R01 NS117604NINDS NIH HHS R25 NS065729
6 · The paper itself

Abstract

Degeneration of the corticospinal tract is a feature in several neurodegenerative disorders and leads to disability. However, modeling corticospinal neuron (CSN) pathology and corticospinal connectivity is challenging, as there are interspecies differences in these networks. We developed a human induced pluripotent stem cell (hiPSC)-based microfluidic platform for modeling human CSN and spinal motor neuron (SpMN) connectivity. The incorporation of regionally specific astrocyte subtypes (cortical and spinal) in addition to CSNs and SpMNs allows for the modeling of neural cell interactions. Multielectrode array electrophysiology reveals the temporal maturation of the network. Retrograde labeling demonstrates synaptic connectivity between CSNs and SpMN. Optogenetic strategies to selectively activate excitatory cortical neurons (CNs) attenuated by glutamate receptor antagonism confirm the functional relevance of the model. Incorporating morphological, electrophysiological, and physiological measures of corticospinal connectivity, this platform is a versatile model for neurodegenerative disease research and the future development of targeted CSN therapies.

Indexed as

Induced Pluripotent Stem CellsLab-On-A-Chip DevicesPyramidal TractsAstrocytesHumansMotor Neuronscortical neuroncorticofugalCP: neuroscienceCP: stem cellmotor neuronstem cells

Identifiers

PMID42161268
PMCPMC13390070

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.