Evidence map›Paper›PMID 42160030›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2026

In Situ Single-Particle LSPR Biosensor for Amplification-Free Detection of miR-451 via Dark-Field Scattering Spectroscopy.

Xingyu Zi, Hongen Cheng, Jianqi Wang, Kuilin Liu, Xinyu Gao, Guohua Liu, Mingqing Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xingyu ZiCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin, China.
Hongen ChengCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin, China.
Jianqi WangCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin, China.
Kuilin LiuCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin, China.
Xinyu GaoDepartment of Colorectal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.
Guohua LiuCollege of Electronic Information and Optical Engineering, Nankai University, Tianjin, China.ORCID https://orcid.org/0000-0003-1619-7576
Mingqing ZhangDepartment of Colorectal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin, China.

Funding

National Natural Science Foundation of China 62471256
6 · The paper itself

Abstract

Colorectal cancer (CRC) is frequently diagnosed at advanced stages due to the asymptomatic nature of early disease and the semi-invasive nature of colonoscopy. Developing effective noninvasive methods for CRC-related biomarker detection is crucial for reducing CRC mortality. Notably, miR-451 has been reported as a CRC-related biomarker candidate. We present an in situ single-particle spectroscopic assay for amplification-free quantitative detection of miR-451 using gold nanoparticles (AuNPs). Functionalized substrates with immobilized AuNPs enable real-time monitoring of localized surface plasmon resonance (LSPR) peak shifts at the single-particle level. By statistically analyzing the proportion of AuNPs exhibiting LSPR shifts, we achieved quantitative detection of miR-451. This in situ microfluidic configuration allows spectral analysis of the same nanoparticles before and after detection, eliminating spatial sampling errors. Specificity is conferred by a stem-loop DNA probe on the AuNP surface via strand displacement. The main advance lies in in situ tracking of the same immobilized nanoparticles with statistical single-particle readout. This strategy demonstrates a detection limit of 58 fm and excellent specificity against other miRNAs, with proof-of-concept feasibility in spiked FBS samples.

Indexed as

Biosensing TechniquesMicroRNAsSurface Plasmon ResonanceGoldHumansMetal NanoparticlesGoldMicroRNAsMIRN451 microRNA, humanAuNPsin situ detectionlocalized surface plasmon resonancemiR‐451

Identifiers

PMID42160030
PMCPMC13360862

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.