Kisspeptin-10 regulates glycosaminoglycan and decorin content in human cardiac fibroblast cultures.

Paulina Radwańska, Maciej Olkiewicz, Lucyna Piera, Jacek Drobnik

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Article in Pharmacological reports : PR, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Paulina RadwańskaDepartment of Pathophysiology, Chair of General and Experimental Pathology, Medical University of Lodz, Żeligowskiego 7/9, Łódź, 90-752, Poland. paulina.radwanska@umed.lodz.pl.ORCID http://orcid.org/0000-0002-1895-8239

Maciej OlkiewiczDepartment of Pathophysiology, Chair of General and Experimental Pathology, Medical University of Lodz, Żeligowskiego 7/9, Łódź, 90-752, Poland.

Lucyna PieraDepartment of Pathophysiology, Chair of General and Experimental Pathology, Medical University of Lodz, Żeligowskiego 7/9, Łódź, 90-752, Poland.

Jacek DrobnikDepartment of Pathophysiology, Chair of General and Experimental Pathology, Medical University of Lodz, Żeligowskiego 7/9, Łódź, 90-752, Poland.

Funding

Narodowe Centrum Nauki 2018/02/X/NZ5/03158Uniwersytet Medyczny w Lodzi 503/6-103-02/503-61-001

6 · The paper itself

Abstract

backgroundCardiac fibroblasts play a key role in extracellular matrix (ECM) remodelling through the synthesis of glycosaminoglycans and proteoglycans (PGs), such as decorin; however, the effects of kisspeptin-10 (KiSS-10) on these components in the heart remain unknown. The aim of this study was to determine the influence of KiSS-10 on glycosaminoglycan and decorin content in human cardiac fibroblast cultures and identify its mechanism.

methodsThe effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor. Glycosaminoglycan levels were determined by the Farndale method, decorin secretion by enzyme-linked immunosorbent assay (ELISA), and decorin expression by quantitative polymerase chain reaction (qPCR).

resultsKiSS-10 treatment caused a dose-dependent increase in glycosaminoglycan content in both cells and medium. This stimulatory effect was abolished by blocking G-protein-coupled receptor 54 (GPR54) with peptide 234 or focal adhesion kinase (FAK) with FAK inhibitor 14 (FAKi). Combining KiSS-10 with phospholipase C inhibitor (D609) did not alter the glycosaminoglycan level compared to KiSS-10 alone. KiSS-10 increased decorin secretion but not expression. Combining peptide 234 with KiSS-10 did not alter media decorin level compared to KiSS-10 alone.

conclusionsKiSS-10 significantly elevated glycosaminoglycan and decorin accumulation in human cardiac fibroblast cultures. This regulatory effect on glycosaminoglycan level appears to be dependent on GPR54 activation and FAK signalling, but not on phospholipase C function. Hence, KiSS-10 may be involved in ECM remodelling in the heart.

Indexed as

DecorinFibroblastsGlycosaminoglycansKisspeptinsMyocardiumCell LineCells, CulturedDose-Response Relationship, DrugExtracellular MatrixHumansReceptors, G-Protein-CoupledSignal TransductionDecorinGlycosaminoglycansKISS1 protein, humanKisspeptinsReceptors, G-Protein-CoupledDecorinGlycosaminoglycanHuman cardiac fibroblastsKisspeptin-10

Identifiers

PMID42159865

DOI10.1007/s43440-026-00870-6

PMCPMC13437659

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.