Evidence map›Paper›PMID 42159345›Full record

ArticleeLife2026

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling.

Stephan Tetenborg, Eyad Shihabeddin, Elizebeth Olive Akansha Manoj Kumar, Crystal L Sigulinsky, Karin Dedek, Ya-Ping Lin, Fabio A Echeverry, Hannah Hoff, Alberto E Pereda, Bryan W Jones and 4 more

Abstract read
In one paragraph

Article in eLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Stephan TetenborgCollege of Optometry, University of Houston, Houston, United States.ORCID https://orcid.org/0000-0002-9025-0446
Eyad Shihabeddin *College of Optometry, University of Houston, Houston, United States.ORCID https://orcid.org/0000-0002-3140-1523
Elizebeth Olive Akansha Manoj Kumar *College of Optometry, University of Houston, Houston, United States.ORCID https://orcid.org/0009-0000-1264-3642
Crystal L Sigulinsky *Moran Eye Center/Ophthalmology, University of Utah, Salt Lake City, United States.ORCID https://orcid.org/0000-0002-5642-8474
Karin DedekAnimal Navigation/ Neurosensorics, Institute for Biology and Environmental Sciences, University of Oldenburg, Oldenburg, Germany.ORCID https://orcid.org/0000-0003-1490-0141
Ya-Ping LinCollege of Optometry, University of Houston, Houston, United States.
Fabio A EcheverryDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, United States.ORCID https://orcid.org/0000-0002-4200-4080
Hannah HoffDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, United States.
Alberto E PeredaDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, United States.ORCID https://orcid.org/0000-0002-8283-8768
Bryan W JonesMoran Eye Center/Ophthalmology, University of Utah, Salt Lake City, United States.ORCID https://orcid.org/0000-0001-5527-6643
Christophe P RibelaygaCollege of Optometry, University of Houston, Houston, United States.ORCID https://orcid.org/0000-0001-5889-2070
Klaus EbnetInstitute-Associated Research Group: Cell Adhesion and Cell Polarity, Institute of Medical Biochemistry, ZMBE, University of Münster, Münster, Germany.ORCID https://orcid.org/0000-0002-0417-7888
Ken MatsuuraCell Signal Unit, Okinawa Institute of Science and Technology, Onna-son, Japan.ORCID https://orcid.org/0000-0003-0247-3867
John O'BrienCollege of Optometry, University of Houston, Houston, United States.ORCID https://orcid.org/0000-0002-0270-3442

Funding

Research Programming ModuleP30EY007551 · NEI · UNIVERSITY OF HOUSTON · PI LAURA J. FRISHMAN · 1988 to 2026
$16.4M
University of Utah, Core Vision Research GrantP30EY014800 · NEI · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI PAUL STEVEN BERNSTEIN · 2005 to 2026
$14.6M
REGULATION OF RETINAL GAP JUNCTIONSR01EY012857 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI JOHN O'BRIEN · 2000 to 2026
$8.3M
SUPPORT FOR THE ROSE F KENNEDY IDDRC P50P50HD105352 · NICHD · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI Julie Secombe · 2021 to 2026
$7.0M
Retinal CircuitryR01EY028927 · NEI · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Bryan William Jones · 2019 to 2026
$5.2M
NRSA Training CoreTL1TR003169 · NCATS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI CHANDRA, JOYA, FROST, JEFFREY A. · 2019 to 2023
$3.7M
Transgenic tools for revealing the contributions of electrical synapses to neural circuitsRF1MH120016 · NIMH · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI MILLER, ADAM C, O'BRIEN, JOHN · 2020 to 2020
$3.1M
Generation of transgenic zebrafish to study electrical synaptic transmissionR21NS085772 · NINDS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI PEREDA, ALBERTO E · 2013 to 2014
$469k
Facilities for research use of fishG20RR024000 · NCRR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI O'BRIEN, JOHN · 2007 to 2007
$220k
Rod photoreceptor regeneration in a zebrafish model of Retinitis Pigmentosa.F31EY034793 · NEI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SHIHABEDDIN, EYAD · 2023 to 2025
$82k
Deutsche Forschungsgemeinschaft TE 1459/1-1National Science Foundation 2014862NCRR NIH HHS G20 RR024000NEI NIH HHS F31 EY034793NEI NIH HHS P30 EY007551NEI NIH HHS R01 EY012857NICHD NIH HHS P50 HD105352NIH Blueprint for Neuroscience Research P30EY014800NIH Blueprint for Neuroscience Research R01EY012857NIH Blueprint for Neuroscience Research R01EY028927NIH Blueprint for Neuroscience Research R21NS085772NIH Blueprint for Neuroscience Research RF1MH120016NIH Core grant P30EY007551NIH Individual grant F31EY034793NIH training grant TL1TR003169NIMH NIH HHS RF1 MH120016NINDS NIH HHS R21 NS085772
6 · The paper itself

Abstract

Electrical synapses containing Connexin 36 (Cx36) represent the main means for direct electrical communication among neurons in the mammalian nervous system. However, little is known about the protein complexes that constitute these synapses. In the present study, we applied different BioID strategies to screen the interactomes of Connexin 36 and its zebrafish orthologue Cx35.1 in retinal neurons. For in vivo proximity labeling in mice, we took advantage of the Cx36-EGFP strain and expressed a GFP-nanobody-TurboID fusion construct selectively in AII amacrine cells. For in vivo BioID in zebrafish, we generated a transgenic line expressing a Cx35.1-TurboID fusion under control of the

Indexed as

ConnexinsElectrical SynapsesProteomeRetinal NeuronsAmacrine CellsAnimalsAnimals, Genetically ModifiedGap Junction delta-2 ProteinMiceZebrafishConnexinsGap Junction delta-2 ProteinProteomeAAVAII amacrine cellsconnexin 36electrical synapsemouseneurosciencesynaptic scaffoldsturboIDzebrafish

Identifiers

PMID42159345
PMCPMC13189625

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.