ArticleBiotechnology and bioengineering2026
IMACulate(DE3), an E. coli Strain for High Purity His-Tagged Protein Purifications.
Article in Biotechnology and bioengineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his-tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co-purified with the target protein. The low background strain LOBSTR-RIL has been previously engineered with mutated forms of SlyD and ArnA that exhibit reduced binding to Ni
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