Evidence map›Paper›PMID 42157218›Full record

ArticleJournal of nanobiotechnology2026

Rational design of topology-defined DNA nanoframeworks for antigen delivery and cross-presentation.

Ying Cao, Yuanyuan Wu, Xian Huang, Kui Huang, Tomasz Maj, Zhao Xu, Jingwei Sun, Qian Shi, Yang Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ying CaoInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Yuanyuan WuInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Xian HuangInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Kui HuangInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Tomasz MajInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Zhao XuGrit Biotechnology Co., Ltd, Shanghai, 200003, China.
Jingwei SunGrit Biotechnology Co., Ltd, Shanghai, 200003, China.
Qian ShiInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. sshiqian@hotmail.com.
Yang YangInstitute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. yang.yang.nano@sjtu.edu.cn.

Funding

Fundamental Research Funds for the Central Universities YG2025QNB37National Natural Science Foundation of China 22277077National Natural Science Foundation of China 22507076Natural Science Foundation of Shanghai 25ZR1402321
6 · The paper itself

Abstract

DNA nanoframeworks (DNFs) provide structurally programmable platforms for antigen delivery, yet how framework topology influences antigen cross-presentation remains unclear. Here, we systematically compare DNFs with distinct geometries and mechanical properties and demonstrate that rigid frameworks exhibit enhanced serum stability and antigen delivery efficiency. Notably, antigen encapsulation within DNFs consistently promotes cross-presentation compared with surface display, despite similar or lower cellular uptake. This behavior is consistent with an encapsulation-protection mechanism that preserves antigen integrity during intracellular processing. While DNF-mediated dendritic cell activation can be readily attained, the efficiency of antigen cross-presentation is primarily determined by antigen availability, which is in turn regulated by framework topology and antigen localization. This establishes antigen delivery as the key bottleneck for subsequent CD8⁺ T cell activation. Collectively, these two factors are identified as key design parameters for optimizing DNA-based antigen delivery systems.

Indexed as

Antigen PresentationAntigensCross-PrimingDNAAnimalsCD8-Positive T-LymphocytesDendritic CellsDNA NanostructuresHumansLymphocyte ActivationMiceAntigensDNAAntigen cross-presentationAntigen encapsulationDendritic cell–mediated immunityDNA nanoframeworksFramework topology

Identifiers

PMID42157218
PMCPMC13377784

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.