Evidence map›Paper›PMID 42157179›Full record

ArticleJournal of translational medicine2026

Blood plasma and oral rinse liquid profiling for human papillomavirus in head and neck cancer - Unmasking false-positive p16 tissue cases and tracking disease dynamics.

Matthias Mack, Romina Roesch, Jakob Rinecker, Fabian Stögbauer, Sara Krippgans, Markus Nieberler, Katharina Püchler, Cecilia Garcia-Perez, Ramona Secci, Anne Jacob and 8 more

Abstract read
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In one paragraph

Article in Journal of translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Matthias Mack *Institute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Romina Roesch *Institute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Jakob RineckerDepartment of Otolaryngology Head and Neck Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Fabian StögbauerInstitute of Pathology, School of Medicine, Technical University of Munich, Munich, Germany.
Sara KrippgansDepartment of Oral and Maxillofacial Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Markus NieberlerDepartment of Oral and Maxillofacial Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Katharina PüchlerInstitute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Cecilia Garcia-PerezDepartment of Otolaryngology Head and Neck Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Ramona SecciInstitute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Anne JacobInstitute of Pathology, School of Medicine, Technical University of Munich, Munich, Germany.
Nicole PfarrInstitute of Pathology, School of Medicine, Technical University of Munich, Munich, Germany.
Katja SteigerInstitute of Pathology, School of Medicine, Technical University of Munich, Munich, Germany.
Carolin MoglerInstitute of Pathology, School of Medicine, Technical University of Munich, Munich, Germany.
Barbara WollenbergDepartment of Otolaryngology Head and Neck Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Jürgen RulandInstitute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Irina A Kerle *Department for Translational Medical Oncology, National Center for Tumor Diseases Dresden (NCT/UCC), a Partnership Between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, and Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany.
Markus Wirth *Department of Otolaryngology Head and Neck Surgery, School of Medicine and Health, Technical University of Munich, Munich, Germany.
Christof Winter *Institute of Clinical Chemistry and Pathobiochemistry, TUM University Hospital, School of Medicine and Health, Technical University of Munich, Munich, Germany. christof.winter@tum.de.ORCID 0000-0002-0253-9056

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman papillomavirus (HPV) is a major etiological factor in a subset of head and neck squamous cell carcinomas (HNSCC), particularly in the oropharynx, and reliable detection is critical for prognosis and treatment decisions.

methodsWe applied droplet digital PCR (ddPCR) assays targeting HPV16 oncogenes (E6, E7) and the non-oncogenic gene E2 to tumor tissue, blood plasma, and oral rinse samples from 58 HNSCC patients. Results were compared with p16

resultsddPCR identified 41% of patients as HPV16-positive in tumor tissue, with higher specificity than p16

conclusionddPCR-based HPV testing provides high diagnostic specificity compared with p16

Indexed as

Cyclin-Dependent Kinase Inhibitor p16Disease ProgressionHead and Neck NeoplasmsHuman Papillomavirus VirusesMouthAgedDNA, ViralFalse Positive ReactionsFemaleHumansImmunohistochemistryLiquid BiopsyMaleMiddle AgedPolymerase Chain ReactionCyclin-Dependent Kinase Inhibitor p16DNA, ViralBiomarkerCell-free DNACirculating tumor DNADiagnostic specificityDroplet digital PCRHead and neck squamous cell carcinomaHuman papillomavirusLiquid biopsyLongitudinal monitoringOral rinseP16 immunohistochemistryPlasma

Identifiers

PMID42157179
PMCPMC13192202

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.