ArticleMethods in molecular biology (Clifton, N.J.)2026
A Comprehensive Approach to Monitor Endocytosis and Trafficking of G Protein-Coupled Receptors: Tools for Exploring Receptor-Lipid Interaction.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
G protein-coupled receptors (GPCRs) are cellular nanomachines that act as signaling hubs and account for ~40% of current drug targets. Endocytosis of GPCRs represents a major regulatory mechanism to sustain their downstream signaling within a stringent spatiotemporal regime. This is achieved by internalizing a fraction of the plasma membrane receptor population, thereby reducing the available pool of receptors for signaling at the cell membrane. In this article, we describe a detailed step-by-step hands-on guide that we have successfully used to monitor GPCR endocytosis and intracellular trafficking using serotonin
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