Evidence map›Paper›PMID 42156686›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers.

Evan L Taylor, Kumud Raj Poudel, James A Brozik

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Evan L TaylorDepartment of Chemistry, Washington State University, Pullman, WA, USA.
Kumud Raj PoudelCMC, Mammoth Biosciences, Brisbane, CA, USA.
James A BrozikDepartment of Chemistry, Washington State University, Pullman, WA, USA. brozik@wsu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this chapter is to serve as a guide for those who wish to carry out experiments tracking single proteins in planar-supported biomimetic membranes. This chapter describes, in detail, the construction of a simple single-molecule microscope, which includes (1) a parts list, (2) temperature control, (3) an alignment procedure, (4) a calibration procedure, and (5) a procedure for measuring the mechanical stability of the instrument. It also gives procedures for making planar-supported bilayers on hydrophilically treated borosilicate and quartz. These include (1) POPC bilayers, (2) POPC/PEG-PE cushioned bilayers, (3) POPC/PEG-PE cushioned bilayers on BSA passivated substrates, and (4) a cushioned biomimetic membrane of the endoplasmic reticulum (ER). A procedure for the detergent-mediated incorporation of the transmembrane protein 5HT

Indexed as

Lipid BilayersMembrane ProteinsSingle Molecule ImagingAnimalsEndoplasmic ReticulumPhosphatidylcholines1-palmitoyl-2-oleoylphosphatidylcholineLipid BilayersMembrane ProteinsPhosphatidylcholinesActivation energiesArrhenius analysisBiomimeticIntegral membrane proteinIn vitro protein studiesPeripheral membrane proteinProtein diffusionSingle molecule instrument designSingle molecule microscopySingle molecule trackingTransmembrane protein

Identifiers

PMID42156686

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.