Purification of Bacterial Lipopolysaccharide, and How to Study Its Interactions with Proteins.

Athanasios Saragliadis, Daniel Hatlem, Dirk Linke

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Athanasios SaragliadisUniversity of Oslo, Department of Biosciences, Blindernveien 31, 0371, Oslo, Norway.ORCID http://orcid.org/0000-0001-7950-6781

Daniel HatlemMax Planck Institute of Biophysics, Max-von-Laue-Str. 3, 60438, Frankfurt am Main, Germany.ORCID http://orcid.org/0000-0001-6945-8675

Dirk LinkeUniversity of Oslo, Department of Biosciences, Blindernveien 31, 0371, Oslo, Norway. dirk.linke@ibv.uio.no.ORCID http://orcid.org/0000-0003-3150-6752

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipopolysaccharides (LPS) are an important component of the outer membrane of Gram-negative bacteria. Although LPS is not homogeneous and it varies among bacterial species and even from strain to strain, there are significantly conserved elements that confer and dictate the physicochemical properties of this molecule. Isolating and purifying LPS from its source organism provides the means to investigate the interactions of LPS molecules with other binding partners, such as proteins. Here, we describe methods to isolate LPS and discuss methods for interaction studies.

Indexed as

Bacterial ProteinsLipopolysaccharidesElectrophoresis, Polyacrylamide GelGram-Negative BacteriaProtein BindingSilver StainingBacterial ProteinsLipopolysaccharidesLipopolysaccharideLPS isolationLPS–protein interactionSDS-PAGESilver-staining

Identifiers

PMID42156675

DOI10.1016/j.mimet.2010.04.002

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.