Evidence map›Paper›PMID 42156649›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Exploring the Ocean's Microbial World: Techniques and Protocols for Microbiome Research.

Vijaya Raghavan Rangamaran, T J Sushmitha, Karthikeyan Kathan Tamilmani, Hinduja Murugesan, Dharani Gopal

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Vijaya Raghavan RangamaranMarine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India. vijay.niot@gov.in.
T J SushmithaMarine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India.
Karthikeyan Kathan TamilmaniMarine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India.
Hinduja MurugesanMarine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India.
Dharani GopalMarine Biotechnology Group, National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (MoES), Government of India, Chennai, India. dharani.niot@gov.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marine microbiomes play a crucial role in oceanic ecosystems, influencing biogeochemical cycles, climate regulation, and marine biodiversity. Accurate characterization of these microbial communities requires standardized protocols for sample collection, processing, sequencing and data analysis. This chapter provides a comprehensive guide to essential methodologies for marine microbiome research including field sampling strategies, DNA and RNA extraction techniques, high-throughput sequencing approaches (such as 16S rRNA amplicon sequencing and metagenomics) and bioinformatics pipelines for data interpretation. Additionally, we discuss quality control measures, best practices for reproducibility, and challenges associated with marine microbiome profiling. By adopting standardized methodologies, researchers can generate reliable, comparable datasets that enhance our understanding of marine microbial ecology and its broader environmental implications.

Indexed as

MetagenomicsMicrobiotaSeawaterBiodiversityComputational BiologyHigh-Throughput Nucleotide SequencingMetagenomeOceans and SeasRNA, Ribosomal, 16SRNA, Ribosomal, 16SBioinformatics analysisMarine microbiomeSampling protocolsWhole metagenome shotgun sequencing

Identifiers

PMID42156649

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.