Evidence map›Paper›PMID 42156577›Full record

ArticlePflugers Archiv : European journal of physiology2026

KCa3.1 mediates radioresistance of silver nanoparticles in human glioblastoma cells.

Francesco Ragonese, Andrea Biagini, Luana Sallicandro, Alessandra Mirarchi, Gianmarco Reali, Antonella De Luca, Cataldo Arcuri, Sara Flamini, Oxana Bereshchenko, Paolo Cogliati and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pflugers Archiv : European journal of physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Francesco Ragonese *Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Andrea Biagini *Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Luana Sallicandro *Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Alessandra Mirarchi *Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Gianmarco Reali *Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Antonella De LucaDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Cataldo ArcuriDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Sara FlaminiDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Oxana BereshchenkoDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Paolo CogliatiDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Roberto SpogliProlabin & Tefarm, Spin-Off University of Perugia, Perugia, 06134, Italy.
Paola SabbatiniDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy.
Stefano BruscoliDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Paolo SportolettiDepartment of Medicine and Surgery, University of Perugia, Perugia, 06132, Italy.
Bernard FiorettiDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy. bernard.fioretti@unipg.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Silver nanoparticles (AgNPs) have emerged as a promising therapeutic tool for managing glioblastoma (GB), particularly in radiosensitisation. However, their mechanisms of action are not yet fully understood. Since ion channel activity is implicated in various aspects of radioresistance, we investigated the electrophysiological effects of AgNPs on GB cells. In the U251 human GB cell line, the acute application of AgNPs induced the activation of a cationic nonselective current in a dose-dependent manner, which was not affected by ACA (TRPM2 blocker). In addition, AgNPs increased intracellular calcium concentration, caused depolarisation of the mitochondrial inner membrane, and promoted membrane blebbing. The investigation revealed that calcium influx is a critical step in the AgNPs-induced effects. Thiol reagents such as cysteine are known silver chelating agents and their application prevented the activation of cationic currents, intracellular calcium increases, and membrane blebbing formation induced by AgNPs. This finding suggested Ag

Indexed as

GlioblastomaIntermediate-Conductance Calcium-Activated Potassium ChannelsMetal NanoparticlesRadiation ToleranceSilverCalciumCell Line, TumorHumansCalciumIntermediate-Conductance Calcium-Activated Potassium ChannelsKCNN4 protein, humanSilverGlioblastomaIntracellular calciumIon ChannelsKCa3.1NanomedicineRadioresistanceSilver nanoparticles

Identifiers

PMID42156577
PMCPMC13186830

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.