Evidence map›Paper›PMID 42155443›Full record

ArticleCell genomics2026

Essential lncRNAs in the human transcriptome.

Wen-Wei Liang, Simon J Mueller, Sydney K Hart, Hans-Hermann Wessels, Alejandro Méndez-Mancilla, Akash Sookdeo, Olivia Choi, Christina M Caragine, Alba Corman, Lu Lu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wen-Wei LiangNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Simon J MuellerNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Sydney K HartNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Hans-Hermann WesselsNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Alejandro Méndez-MancillaNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Akash SookdeoNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Olivia ChoiNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Christina M CaragineNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Alba CormanNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Lu LuNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Olena KolumbaNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Breanna WilliamsNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA.
Neville E SanjanaNew York Genome Center, New York, NY 10013, USA; Department of Biology, New York University, New York, NY 10013, USA. Electronic address: neville@sanjanalab.org.

Funding

Predicting the functional impact of non-coding mutations using patient-specific gene regulatory networksR01CA218668 · NCI · WEILL MEDICAL COLL OF CORNELL UNIV · PI EKTA KHURANA, Neville Sanjana · 2018 to 2026
$4.7M
Multiscale genome engineering to map cis-regulatory variants in human and mouseR01HG012790 · NHGRI · NEW YORK GENOME CENTER · PI Tuuli Lappalainen, Dan Littman · 2023 to 2026
$4.5M
In situ functional genomics to understand transcriptional regulationDP2HG010099 · NHGRI · NEW YORK GENOME CENTER · PI SANJANA, NEVILLE · 2017 to 2022
$4.4M
Enhancing Susceptibility of HIV Reservoirs to CTL Through a Discovery to Translational ApproachR01AI176601 · NIAID · WEILL MEDICAL COLL OF CORNELL UNIV · PI R. Brad Jones, Neville Sanjana · 2023 to 2026
$3.3M
LTBR CARs as next-generation therapies for R/R lymphomaR01CA279135 · NCI · NEW YORK GENOME CENTER · PI Catherine Sibyl Diefenbach, Neville Sanjana · 2023 to 2026
$3.2M
Scalable multi-ancestry functional genomics of blood traits and cardiovascular diseaseR01HL168247 · NHLBI · NEW YORK GENOME CENTER · PI Neville Sanjana · 2024 to 2026
$2.4M
NCI NIH HHS R01 CA218668NCI NIH HHS R01 CA279135NHGRI NIH HHS DP2 HG010099NHGRI NIH HHS R01 HG012790NHLBI NIH HHS R01 HL168247NIAID NIH HHS R01 AI176601
6 · The paper itself

Abstract

Mammalian genomes host a diverse array of RNAs, including protein-coding and noncoding transcripts. However, the functional roles of most long noncoding RNAs (lncRNAs) remain elusive. Using RNA-targeting CRISPR-Cas13 screens, we probed how the loss of ∼5,500 lncRNAs impacts cell fitness across five human cell lines and identified 788 lncRNAs with context-specific or broad essentiality. We confirm their essentiality through individual perturbations and find that the majority of essential lncRNAs operate independently of their nearest protein-coding genes. Using transcriptome profiling in single cells, we discover that loss of essential lncRNAs impairs cell cycle progression and drives apoptosis. Many essential lncRNAs demonstrate dynamic expression across tissues during development. Using ∼9,000 primary tumors, we pinpoint those lncRNAs whose expression in tumors correlates with survival, yielding new biomarkers and potential therapeutic targets. This transcriptome-wide survey of functional lncRNAs advances our understanding of noncoding transcripts and demonstrates the potential of transcriptome-scale noncoding screens with Cas13.

Indexed as

RNA, Long NoncodingTranscriptomeApoptosisCell CycleCell Line, TumorCRISPR-Cas SystemsGene Expression ProfilingGene Expression Regulation, NeoplasticHumansNeoplasmsRNA, Long NoncodingCRISPR-Cas13essential geneshuman developmentlncRNAMALAT1MIR17HGNoncoding RNARNA targetingtranscriptome-scaletumor biomarkers

Identifiers

PMID42155443
PMCPMC13347947

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.