Evidence map›Paper›PMID 42154657›Full record

ArticleACS synthetic biology2026

An Oxygen-Tolerant Archaeal Dehydratase Enables a Synthetic Pathway for 4-Hydroxybutyrate-Containing Polyesters from Glucose and CO

Kai-Hee Huong, Shizuru Ishihara, Izumi Orita, Toshiaki Fukui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kai-Hee HuongSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Shizuru IshiharaSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Izumi OritaSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Toshiaki FukuiSchool of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.ORCID 0000-0003-4557-6284

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

4-Hydroxybutyrate (4HB) and the related compounds are important in the chemical industry, and microbial metabolic engineering for 4HB production has been pioneered via the TCA cycle route employing clostridial enzymes. This study focused on an alternative, energy-efficient route for 4HB-CoA production using a unique enzyme from a different domain of life. The [4Fe-4S] cluster-containing 4HB-CoA dehydratase (4HcD), catalyzing radical-based atypical dehydration/hydration, is one of the enzymes difficult to apply in biotechnological applications due to its high sensitivity to reactive oxygen species. The present results demonstrated that an oxygen-tolerant 4HcD derived from an aerobic archaeon enabled conversion of crotonyl-CoA to 4HB-CoA in polyhydroxyalkanoate (PHA)-producing

Indexed as

Carbon DioxideCupriavidus necatorGlucoseHydro-LyasesHydroxybutyratesOxygenPolyestersAcyl Coenzyme AMetabolic EngineeringAcyl Coenzyme ACarbon Dioxidecrotonyl-coenzyme AGlucoseHydro-LyasesHydroxybutyratesOxygenPolyesters4-hydroxybutyrateArchaeal dehydrataseCupriavidus necatoroxygen-tolerantpoly(3-hydroxybutyrate-co-4-hydroxybutyrate)polyhydroxyalkanoates

Identifiers

PMID42154657
PMCPMC13288912

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.