Evidence map›Paper›PMID 42154598›Full record

ArticleSTAR protocols2026

Protocol for inducing cutaneous squamous cell carcinoma with chronic UV and treating with 5-aminolevulinic acid photodynamic therapy in SKH-1 mice.

Wenbo Zhou, Keni Chang, Zhijie Ruan, Lunhui Chang, Xiaoying Gao, Xuan Liu, Guorong Yan, Xiuli Wang, Guolong Zhang, Qingyu Zeng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wenbo ZhouInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Keni ChangInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Zhijie RuanInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Lunhui ChangInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China; The First Hospital of Anhui University of Science and Technology, Huainan 232007, China.
Xiaoying GaoInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China; The First Hospital of Anhui University of Science and Technology, Huainan 232007, China.
Xuan LiuInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Guorong YanInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Xiuli WangInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China.
Guolong ZhangInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address: glzhangtj@tongji.edu.cn.
Qingyu ZengInstitute of Photomedicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200092, China; Skin Cancer Center, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address: zengqingyu2011@tongji.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for establishing cutaneous squamous cell carcinoma (cSCC) in SKH-1 hairless mice using a chronic ultraviolet irradiation regimen, followed by treatment with topical 5-aminolevulinic acid photodynamic therapy (ALA-PDT). We describe steps for a 28-week UV induction schedule, standardized preparation and application of ALA cream, and precise parameters for light irradiation. The model recapitulates key stages of human photocarcinogenesis, providing a physiologically relevant system for investigating cSCC pathogenesis and assessing PDT efficacy in vivo. For complete details on the use and execution of this protocol, please refer to Chen et al.

Indexed as

Aminolevulinic AcidCutaneous Squamous Cell CarcinomaPhotochemotherapySkin NeoplasmsUltraviolet RaysAnimalsDisease Models, AnimalHumansMiceMice, HairlessPhotosensitizing AgentsAminolevulinic AcidPhotosensitizing AgentsCancerClinical ProtocolModel Organisms

Identifiers

PMID42154598
PMCPMC13213768

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.