ArticleMicrobiology spectrum2026
Comparison of diagnostic accuracy and sensitivity of Kinyoun and auramine-rhodamine stains in detecting mycobacteria from specimen smears.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Smear microscopy using carbolfuschin- or auramine-based staining is widely employed for rapid mycobacterial detection from patient specimens. While auramine stains are preferred to detect IMPORTANCE: Accurate and rapid detection of mycobacteria is essential for timely patient management, especially in vulnerable immunocompromised patients. This study analyzes more than 16,000 clinical specimens over 8 years and provides a direct comparison of auramine rhodamine and Kinyoun stains for mycobacterial detection on smear microscopy. Both stains show high specificity and comparable overall performance, but Kinyoun exhibits a modest trend toward improved the detection of rapid growing mycobacteria. These findings offer guidance for clinical laboratories performing smear microscopy for both tubercular and non-tuberculous mycobacteria.
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