Evidence map›Paper›PMID 42152677›Full record

ArticleNucleic acids research2026

Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) maintains muscle progenitor identity by stabilizing the Ppp1r1b-lncRNA-PRC2 complex.

Xuedong Kang, Yan Zhao, Stanley F Nelson, April Pyle, Aldons J Lusis, Marlin Touma

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In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Xuedong KangDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095,United States.
Yan ZhaoDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States.
Stanley F NelsonDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095,United States.
April PyleMicrobiology, Immunology & Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States.
Aldons J LusisDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States.
Marlin ToumaDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095,United States.ORCID 0000-0002-2827-4068

Funding

Novel Gene-Environment Regulatory Circuit in Chamber-Specific Growth of Perinatal HeartR01HL153853 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TOUMA, MARLIN · 2020 to 2023
$1.6M
Children's Discovery and Innovation InstituteCongressionally Directed Medical Research Programs HT9425-25-1-0763NHLBI NIH HHS R01 HL153853UCLA Academic Senate Faculty Research Fund
6 · The paper itself

Abstract

Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) is a multifunctional RNA-binding protein of the hnRNP family, yet its role in long non-coding RNA (lncRNA)-mediated epigenetic regulation during myogenesis remains unclear. The lncRNA Ppp1r1b-lncRNA is an established regulator of myogenesis that functions through interaction with Polycomb repressive complex 2 (PRC2) at myogenic gene promoters. Here, we investigated the role of hnRNPA1 in Ppp1r1b-lncRNA-mediated regulation of myogenesis. Fluorescence in situ hybridization (FISH) revealed the subcellular localization of Ppp1r1b-lncRNA, and RNA pulldown coupled with mass spectrometry identified associated proteins. Both hnRNPA1 and EZH2 were found to bind Ppp1r1b-lncRNA, but at distinct regions. Knockdown of hnRNPA1 in mouse C2C12 myoblasts reduced the interaction between Ppp1r1b-lncRNA and EZH2, as determined by RNA immunoprecipitation (RIP), decreased promoter occupancy of Ppp1r1b-lncRNA, as assessed by chromatin isolation by RNA purification (CHIRP), and reduced H3K27me3 levels at the MyoD1 and Myogenin promoters, as shown by chromatin immunoprecipitation (ChIP). These changes led to increased expression of muscle-specific transcription factors and sarcomeric genes, thereby disrupting the undifferentiated state. Furthermore, hnRNPA1 knockdown disrupted the interaction between the human ortholog PPP1R1B-lncRNA and PRC2 in human skeletal muscle precursor cells (hSMPCs). Together, these findings demonstrate that hnRNPA1 maintains the integrity of the Ppp1r1b-lncRNA-PRC2 complex and ensures proper epigenetic regulation of myogenic gene expression. This conserved hnRNPA1-Ppp1r1b-lncRNA-PRC2 regulatory axis represents a potential therapeutic target for muscle regeneration.

Indexed as

Heterogeneous Nuclear Ribonucleoprotein A1Muscle DevelopmentPolycomb Repressive Complex 2RNA, Long NoncodingAnimalsCell DifferentiationCell LineEnhancer of Zeste Homolog 2 ProteinEpigenesis, GeneticHistonesHumansMiceMyoblastsMyoD ProteinPromoter Regions, GeneticEnhancer of Zeste Homolog 2 ProteinHeterogeneous Nuclear Ribonucleoprotein A1HistonesHnrnpa1 protein, mouseMyoD ProteinPolycomb Repressive Complex 2RNA, Long Noncoding

Identifiers

PMID42152677
PMCPMC13183673

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.