Evidence map›Paper›PMID 42152627›Full record

ArticleChemistry & biodiversity2026

Direct Conversion of Phytosterol to Testosterone via an Integrated Microbial-Enzymatic Strategy.

Shikui Song, Xirenayi Tuergong, Zihan Ding, Xinlin Dong, Wang Lang, Zhengding Su

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry & biodiversity, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shikui SongXinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Sciences, Xinjiang Normal University, Urumqi, Xinjiang, China.ORCID https://orcid.org/0009-0005-9795-4859
Xirenayi TuergongXinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Sciences, Xinjiang Normal University, Urumqi, Xinjiang, China.
Zihan DingXinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Sciences, Xinjiang Normal University, Urumqi, Xinjiang, China.
Xinlin DongProtein Engineering and Biopharmaceutical Sciences Laboratory, Hubei University of Technology, Wuhan, Hubei, China.
Wang LangInstitute of Materia Medica, School of Pharmaceutical Sciences, Xinjiang University, Urumqi, Xinjiang, China.
Zhengding SuProtein Engineering and Biopharmaceutical Sciences Laboratory, Hubei University of Technology, Wuhan, Hubei, China.ORCID https://orcid.org/0000-0003-3558-001X

Funding

Doctoral Research Foundation of Xinjiang Normal University XJNUZBS2421National Natural Science Foundation of China 22468047Natural Science Foundation of Xinjiang Uygur Autonomous Region 2024D01B63"Tianchi Talents" introduction plan of Xinjiang Uygur Autonomous RegionXinjiang University Double-First Rate Initiative Program
6 · The paper itself

Abstract

Testosterone is an essential androgenic steroid traditionally produced from 4-androstenedione through multistep chemical or biocatalytic processes. Here, we report a streamlined and sustainable "two-stage" bioconversion strategy for direct testosterone production from phytosterols. In the first stage, Mycobacterium neoaurum NRRL B-3805 was metabolically reprogrammed to maximize 4-androstenedione accumulation by deleting kstd1 and kshB1 and overexpressing hsd4A, generating strain MnB

Indexed as

MycobacteriaceaePhytosterolsTestosteroneMolecular Docking SimulationOxidoreductasesOxidoreductasesPhytosterolsTestosterone4‐androstenedioneketoreductaseMycobacterium neoaurumphytosteroltestosterone

Identifiers

PMID42152627
PMCPMC13376716

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.