Defining Microbiome Impact on Host Physiology During Spaceflight Using Caenorhabditis elegans.

Dana Blackburn, Bushra Rahman, Atiyya P Saroyia, Audrey J Parish, Monica Driscoll, Nathaniel J Szewczyk, Siva A Vanapalli, Buck S Samuel

Abstract read

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dana Blackburn *Alkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.

Bushra Rahman *Department of Chemical Engineering, Texas Tech University, Lubbock, TX, USA.

Atiyya P SaroyiaDepartment of Chemical Engineering, Texas Tech University, Lubbock, TX, USA.

Audrey J ParishAlkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.

Monica DriscollDepartment of Molecular Biology and Biochemistry, Rutgers University, New Brunswick, NJ, USA.

Nathaniel J SzewczykOhio Musculoskeletal and Neurologic Institute and Department of Biomedical Sciences, Ohio University, Athens, OH, USA.

Siva A VanapalliDepartment of Chemical Engineering, Texas Tech University, Lubbock, TX, USA.

Buck S SamuelAlkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA. buck.samuel@bcm.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbiome-integrated Caenorhabditis elegans cultivation methods enable investigation of host-microbiome interactions in the context of space-relevant stresses using three key innovations: introduction of live bacterial communities replacing chemically defined media, implementation of auxin-inducible degradation systems to prevent progeny production, and development of complementary hardware platforms. Polyethylene bags provide gas-permeable cultivation environments for large populations with complex microbiomes supporting downstream molecular analyses, while NemaCapsules with micropillar arrays and passive culturing chambers allow real-time phenotypic assessment through on-orbit imaging, transforming our ability to correlate molecular signatures with physiological outcomes in microgravity.

Indexed as

Caenorhabditis elegansHost Microbial InteractionsMicrobiotaSpace FlightAnimalsWeightlessnessGeneticsHost–microbe interactionsMicrofluidicsMicrogravitySpace health

Identifiers

PMID42151682

DOI10.1007/978-1-0716-0592-9_12

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.