Evidence map›Paper›PMID 42151476›Full record

ArticleCommunications biology2026

SAPCD2 phosphorylation modulates astral microtubule stability to control spindle orientation.

Shuang Chen, Ying Hong, Yaping Fang, Mingxuan Li, Jianhong Xia, Jikai Wen, Feiyang Dai, Yuekai Li, Wandi Bu, Michael S Y Huen and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shuang ChenState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Ying HongState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Yaping FangState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Mingxuan LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Jianhong XiaCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Jikai WenState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.ORCID http://orcid.org/0000-0002-4010-4416
Feiyang DaiState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Yuekai LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Wandi BuState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China.
Michael S Y HuenSchool of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.ORCID http://orcid.org/0000-0003-1774-2425
Jun JiangState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China. jiangjun@scau.edu.cn.ORCID http://orcid.org/0000-0003-3641-3280
Yiqun DengState Key Laboratory of Swine and Poultry Breeding Industry, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, China. yqdeng@scau.edu.cn.ORCID http://orcid.org/0000-0002-6838-3779

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Proper spindle orientation is essential for organogenesis and tissue homeostasis. Cortical tethering complexes, such as the Gαi-LGN-NuMA complex, anchor astral microtubules to guide spindle orientation. However, how astral microtubule stability is coupled to mitosis-dependent regulatory mechanisms remains unclear. Here, we identified suppressor APC domain-containing 2 (SAPCD2) as a cell cycle-regulated factor that functionally links astral microtubule stability to spindle orientation control, utilizing non-polarized HeLa and HaCaT cell culture models. Live-cell imaging revealed that siRNA-mediated depletion of SAPCD2 induces spindle misorientation, leading to mitotic delay and aberrant cell division. Mechanistically, SAPCD2 acts as a previously uncharacterized microtubule-associated protein that promotes microtubule stability, whereas CDK1-mediated phosphorylation at S157, together with phosphorylation at S276, generates a mitosis-specific phosphorylated isoform that destabilizes astral microtubules. Importantly, disruption of SAPCD2 phosphorylation at both S157 and S276 fails to rescue the spindle misorientation caused by SAPCD2 depletion. These findings deepen our understanding of how mitosis-dependent dynamic phosphorylation of microtubule-associated proteins orchestrates astral microtubule behavior to ensure accurate spindle orientation.

Indexed as

Microtubule-Associated ProteinsMicrotubulesSpindle ApparatusHeLa CellsHumansMitosisPhosphorylationMicrotubule-Associated Proteins

Identifiers

PMID42151476
PMCPMC13443627

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.